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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Protease phenotype of constitutive connective tissue and of induced mucosal mast cells in mice is regulated by the tissue
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Protease phenotype of constitutive connective tissue and of induced mucosal mast cells in mice is regulated by the tissue

机译：组成性结缔组织和小鼠黏膜肥大细胞的蛋白酶表型受组织调控

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摘要

Mouse mast cells (MCs) express a large number of serine proteases including tryptases, mouse mast cell protease (mMCP)-6 and -7; chymases, mMCP-1, -2, and -4; and an elastase, mMCP-5; along with carboxypeptidase-A3 (CPA3). In helminth-infected mouse intestine, distinct protease phenotypes are observed for connective tissue MCs (CTMCs) (mMCP-4~+-7~+, and CPA3~+) and mucosal MCs (MMCs) (mMCP-1~+ and 2~+). To determine whether the protease phenotype was regulated by the tissue, we compared the phenotype of constitutive CTMCs and induced MMCs in trachea and large airways in antigen-sensitized unchallenged and challenged mice to MCs in skin and helminthic-infected intestine. We found that in the trachea, unlike in skin and intestine, CTMCs and MMCs both express all six serine proteases and CPA3 (mMCP-1~+, -2~+, 4~+-7~+, CPA3~+). This phenotype also holds for the lung CTMCs in the proximal bronchi, whereas the induced MMCs express only four proteases, mMCP-1, -2, -6, and -7. Thus, the T-cell-dependent induction of MMCs in trachea, large bronchi, and small intestine provides numbers but does not determine the protease phenotype. Furthermore, the CTMCs, which are constitutive, also show striking differences at these tissue sites, supporting the view that the differences in expression are tissue directed and not dependent on inflammation.
机译：小鼠肥大细胞（MCs）表达大量丝氨酸蛋白酶，包括类胰蛋白酶，小鼠肥大细胞蛋白酶（mMCP）-6和-7；乳糜，mMCP-1，-2和-4；和弹性蛋白酶，mMCP-5；以及羧肽酶A3（CPA3）。在蠕虫感染的小鼠肠道中，结缔组织MC（CTMC）（mMCP-4〜+ -7〜+和CPA3〜+）和粘膜MC（MMC）（mMCP-1〜+和2〜 +）。为了确定蛋白酶表型是否受组织调控，我们比较了抗原敏感的未挑战和挑战小鼠的气管和大气道中本构性CTMCs和诱导型MMCs与皮肤和蠕虫感染肠中MCs的表型。我们发现，在气管中，与皮肤和肠道不同，CTMC和MMC都表达全部六个丝氨酸蛋白酶和CPA3（mMCP-1〜，-2〜+，4〜+ -7〜+，CPA3〜+）。该表型也适用于近端支气管中的肺部CTMC，而诱导的MMC仅表达四种蛋白酶，即mMCP-1，-2，-6和-7。因此，气管，大支气管和小肠中MMC的T细胞依赖性诱导可提供数量，但不能决定蛋白酶的表型。此外，组成性的CTMC在这些组织部位也显示出惊人的差异，支持以下观点：表达差异是组织定向的且不依赖于炎症。

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    Wei Xing; K. Frank Austen; Michael F. Gurish; Tatiana G. Jones;

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    Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Department of Medicine, Harvard Medical School, Boston, MA 02115;

    Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Department of Medicine, Harvard Medical School, Boston, MA 02115;

    Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Department of Medicine, Harvard Medical School, Boston, MA 02115;

    Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Department of Medicine, Harvard Medical School, Boston, MA 02115;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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