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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Three-dimensional structure and function of the Paramecium bursaria chlorella virus capsid
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Three-dimensional structure and function of the Paramecium bursaria chlorella virus capsid

机译：草履虫法球菌小球藻病毒衣壳的三维结构和功能

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摘要

A cryoelectron microscopy 8.5 A resolution map of the 1,900 A diameter, icosahedral, internally enveloped Paramecium bursaria chlorella virus was used to interpret structures of the virus at initial stages of cell infection. A fivefold averaged map demonstrated that two minor capsid proteins involved in stabilizing the capsid are missing in the vicinity of the unique vertex. Reconstruction of the virus in the presence of host chlorella cell walls established that the spike at the unique vertex initiates binding to the cell wall, which results in the enveloped nucleocapsid moving closer to the cell. This process is concurrent with the release of the internal viral membrane that was linked to the capsid by many copies of a viral membrane protein in the mature infectous virus. Simultaneously, part of the trisymmetrons around the unique vertex disassemble, probably in part because two minor capsid proteins are absent, causing Paramecium bursaria chlorella virus and the cellular contents to merge, possibly as a result of enzyme(s) within the spike assembly. This may be one of only a few recordings of successive stages of a virus while infecting a eukaryotic host in pseudoatomic detail in three dimensions.
机译：冷冻电子显微镜8.5直径为1,900 A的二十面体内部包裹的草履虫法球藻小球藻病毒的分辨率图用于解释细胞感染初期的病毒结构。五倍的平均图谱表明，在唯一顶点附近缺少与稳定衣壳有关的两个次要衣壳蛋白。在宿主小球藻细胞壁存在下病毒的重建证实，独特顶点处的尖峰开始结合细胞壁，这导致被包裹的核衣壳移近细胞。该过程与内部病毒膜的释放同时发生，该内部病毒膜通过成熟感染病毒中病毒膜蛋白的许多拷贝与衣壳连接。同时，独特顶点周围的部分三共通体分解，可能部分是由于缺少两个较小的衣壳蛋白，从而导致了草履虫草小球藻病毒和细胞内物质合并，这可能是由于穗组装中的酶所致。这可能是病毒连续阶段感染伪核细节的三维记录中仅有的几个记录之一。

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  • 作者

    Xinzheng Zhang; Ye Xiang; David D. Dunigan; Thomas Klose; Paul R. Chipman; James L. Van Etten; Michael G. Rossmann;

  • 作者单位

    Department of Biological Sciences, Purdue University, 240 South Martin Jischke Drive, West Lafayette, IN 47907-2032;

    Department of Biological Sciences, Purdue University, 240 South Martin Jischke Drive, West Lafayette, IN 47907-2032;

    Department of Plant Pathology and Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583-0900;

    Department of Biological Sciences, Purdue University, 240 South Martin Jischke Drive, West Lafayette, IN 47907-2032;

    Department of Biological Sciences, Purdue University, 240 South Martin Jischke Drive, West Lafayette, IN 47907-2032;

    Department of Plant Pathology and Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583-0900;

    Department of Biological Sciences, Purdue University, 240 South Martin Jischke Drive, West Lafayette, IN 47907-2032;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    3d structure; cell entry; conformation changes; minor proteins; pbcv-1;

    机译：3d结构;细胞进入;构象变化;次要蛋白质pbcv-1;

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