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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Distant Mimivirus relative with a larger genome highlights the fundamental features of Megaviridae
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Distant Mimivirus relative with a larger genome highlights the fundamental features of Megaviridae

机译：具有较大基因组的远距离Mimivirus突出了Megaviridae的基本特征

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摘要

Mimivirus, a DNA virus infecting acanthamoeba, was for a long time the largest known virus both in terms of particle size and gene content. Its genome encodes 979 proteins, including the first four aminoacyl tRNA synthetases (ArgRS, CysRS, MetRS, and TyrRS) ever found outside of cellular organisms. The discovery that Mimivirus encoded trademark cellular functions prompted a wealth of theoretical studies revisiting the concept of virus and associated large DNA viruses with the emergence of early eukaryotes. However, the evolutionary significance of these unique features remained impossible to assess in absence of a Mimivirus relative exhibiting a suitable evolutionary divergence. Here, we present Megavirus chilensis, a giant virus isolated off the coast of Chile, but capable of replicating in fresh water acanthamoeba. Its 1,259,197-bp genome is the largest viral genome fully sequenced so far. It encodes 1,120 putative proteins, of which 258 (23%) have no Mimivirus homologs. The 594 Megavirus/Mimivirus orthologs share an average of 50% of identical residues. Despite this divergence, Megavirus retained all of the genomic features characteristic of Mimivirus, including its cellular-like genes. Moreover, Megavirus exhibits three additional aminoacyl-tRNA synthetase genes (lleRS, TrpRS, and AsnRS) adding strong support to the previous suggestion that the Mimivirus/Megavirus lineage evolved from an ancestral cellular genome by reductive evolution. The main differences in gene content between Mimivirus and Megavirus genomes are due to (i) lineages specific gains or losses of genes, (ii) lineage specific gene family expansion or deletion, and [Hi) the insertion/migration of mobile elements (intron, intein).
机译：就颗粒大小和基因含量而言，微小病毒是一种感染棘阿米巴的DNA病毒，长期以来一直是已知最大的病毒。它的基因组编码979种蛋白质，包括在细胞有机体之外发现的前四种氨酰基tRNA合成酶（ArgRS，CysRS，MetRS和TyrRS）。 Mimivirus编码商标细胞功能的发现促使大量的理论研究重新探讨了病毒和相关的大型DNA病毒的概念，以及早期的真核生物的出现。但是，在没有表现出合适的进化差异的拟南芥病毒亲戚的情况下，这些独特特征的进化意义仍然无法评估。在这里，我们介绍了巨型病毒chilensis，这是一种在智利海岸附近分离的巨型病毒，但能够在淡水中棘阿米巴中复制。其1,259,197-bp的基因组是迄今为止最大的完全测序的病毒基因组。它编码1,120种推定蛋白质，其中258种（23％）没有拟态病毒同源物。 594个Megavirus / Mimivirus直系同源物平均共享相同残基的50％。尽管存在这种分歧，但Megavirus保留了Mimivirus的所有基因组特征，包括其细胞样基因。此外，Megavirus展示了三个其他的氨酰基-tRNA合成酶基因（lleRS，TrpRS和AsnRS），为先前的观点提供了有力的支持，即Mimivirus / Megavirus谱系通过还原进化从祖先的细胞基因组进化而来。 Mimivirus和Megavirus基因组之间基因含量的主要差异是由于（i）谱系特有的基因得失或（ii）谱系特有的基因家族的扩增或缺失，以及[Hi）流动元件（内含子， intein）。

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    Defne Arslan; Matthieu Legendre; Virginie Seltzer; Chantal Abergel; Jean-Michel Claverie;

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    Information Genomique et Structurale, Centre National de la Recherche Scientifique-Unite Propre de Recherche 2589, Aix-Marseille University, Institut de Microbiologie de la Mediterranee, Pare Scientifique de Luminy, Case 934, FR-13288 Marseille, France;

    Information Genomique et Structurale, Centre National de la Recherche Scientifique-Unite Propre de Recherche 2589, Aix-Marseille University, Institut de Microbiologie de la Mediterranee, Pare Scientifique de Luminy, Case 934, FR-13288 Marseille, France;

    Information Genomique et Structurale, Centre National de la Recherche Scientifique-Unite Propre de Recherche 2589, Aix-Marseille University, Institut de Microbiologie de la Mediterranee, Pare Scientifique de Luminy, Case 934, FR-13288 Marseille, France;

    Information Genomique et Structurale, Centre National de la Recherche Scientifique-Unite Propre de Recherche 2589, Aix-Marseille University, Institut de Microbiologie de la Mediterranee, Pare Scientifique de Luminy, Case 934, FR-13288 Marseille, France;

    Information Genomique et Structurale, Centre National de la Recherche Scientifique-Unite Propre de Recherche 2589, Aix-Marseille University, Institut de Microbiologie de la Mediterranee, Pare Scientifique de Luminy, Case 934, FR-13288 Marseille, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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