Measuring single-enzyme activity in solution

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Measuring single-enzyme activity in solution

机译：测量溶液中的单酶活性

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Measurements of single-enzyme activity allow researchers to understand an enzyme's mode of action, enabling observation of transient intermediates, conformational changes, and the determination of rate constants. Such information can be difficult to obtain because Brownian motion prevents solution-phase molecui from staying still for the length of time required to make meaningful measurements, and immobilization can alter an enzyme's biochemical properties. Randall Goldsmith et al.

机译：通过测量单酶活性，研究人员可以了解酶的作用方式，从而可以观察到瞬时中间体，构象变化以及确定速率常数。由于布朗运动会阻止溶液相分子在进行有意义的测量所需的时间内保持静止，并且固定化会改变酶的生化特性，因此此类信息可能很难获得。 Randall Goldsmith等。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2011年第42期|p.17237|共1页
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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Measuring single-enzyme activity in solution

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