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Refining the DNA barcode for land plants

机译：完善陆地植物的DNA条码

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The goal of DNA barcoding is conceptually simple: Find one or a few regions of DNA that will distinguish among the majority of the world's species, and sequence these from diverse sample sets to produce a large-scale reference library of life on earth (1). This approach can then be used as a tool for species identification and to help in the discovery of new species (2). Since the first DNA barcoding study in 2003 (1), the "animal barcode," a portion of the mitochondrial gene Cytochrome Oxidase 1, has proved remarkably effective at discriminating among species in diverse groups such as birds, fishes, and insects. In contrast, finding a robust and effective barcode for plants has been more difficult. In 2009, a large consortium of researchers, the "Consortium for the Barcode of Life (CBOL) Plant Working Group," proposed portions of two coding regions from the plastid (chloroplast) genome—rbcL and matK—as a "core barcode" for plants, to be supplemented with additional regions as required (3). This recommendation was accepted by the international Consortium for the Barcode of Life, but with the important qualifier that further sequencing of additional markers should be undertaken during a "trial period" (4). This trial period was driven by concerns that routine use of a third (or even a fourth) marker may be necessary to obtain adequate discriminatory power and to guard against sequencing failure for one of the markers (matK can be difficult to amplify and sequence). In PNAS, the China Plant Barcode of Life (BOL) Group provides an impressive dataset tackling this question (5) and assesses the potential benefits of supplementing the core barcode for land plants.
机译：DNA条形码的目标从概念上讲很简单：找到一个或几个可以区分世界上大多数物种的DNA区域，然后对来自不同样本集的这些序列进行测序，以建立一个大规模的地球生命参考库（1） 。然后，该方法可以用作物种识别的工具，并有助于发现新物种（2）。自2003年首次进行DNA条码研究以来（1），“动物条码”是线粒体基因细胞色素氧化酶1的一部分，已被证明在区分鸟类，鱼类和昆虫等不同种类的物种方面非常有效。相比之下，要找到一种健壮有效的植物条形码更为困难。 2009年，一个庞大的研究人员财团“生命条形码组织（CBOL）植物工作组”提出了质体（叶绿体）基因组的两个编码区域rbcL和matK的一部分，作为“核心条形码”植物，并根据需要添加其他区域（3）。该建议已被国际生命条形码协会所接受，但具有重要的限定条件，即应在“试用期内”对其他标记进行进一步的测序（4）。由于担心可能需要常规使用第三（甚至第四）标记以获得足够的区分力并防止其中一种标记的测序失败（matK可能难以扩增和测序），因此该试验期受到关注。在PNAS中，中国植物生命条形码（BOL）组提供了一个令人印象深刻的数据集来解决这个问题（5），并评估了补充陆地植物核心条形码的潜在益处。

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