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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >X-ray structure of the arenavirus glycoprotein GP2 in its postfusion hairpin conformation
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X-ray structure of the arenavirus glycoprotein GP2 in its postfusion hairpin conformation

机译：融合后发夹构象的沙粒病毒糖蛋白GP2的X射线结构

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摘要

Arenaviruses are important agents of zoonotic disease worldwide. The virions expose a tripartite envelope glycoprotein complex at their surface, formed by the glycoprotein subunits GP1, GP2 and the stable signal peptide. This complex is responsible for binding to target cells and for the subsequent fusion of viral and host-cell membranes for entry. During this process, the acidic environment of the endosome triggers a fusogenic conformational change in the transmembrane GP2 subunit of the complex. We report here the crystal structure of the recombinant GP2 ectodomain of the lymphocytic choriomeningitis virus, the arenavirus type species, at 1.8-A resolution. The structure shows the characteristic trimeric coiled coil present in class I viral fusion proteins, with a central stutter that allows a close structural alignment with most of the available structures of class I and III viral fusion proteins. The structure further shows a number of intrachain salt bridges stabilizing the postfusion hairpin conformation, one of which involves an aspartic acid that appears released from a critical interaction with the stable signal peptide upon low pH activation.
机译：禽流感病毒是全世界人畜共患病的重要病原体。病毒体在其表面暴露由糖蛋白亚基GP1，GP2和稳定信号肽形成的三方包膜糖蛋白复合物。该复合物负责与靶细胞结合，并随后使病毒膜和宿主细胞膜融合以进入。在此过程中，内体的酸性环境会触发复合物跨膜GP2亚基的融合构象变化。我们在这里报告的淋巴细胞脉络膜脑膜炎病毒，沙粒病毒类型的物种，在1.8 A分辨率的重组GP2胞外域的晶体结构。该结构显示出I类病毒融合蛋白中存在的特征性三聚体卷曲螺旋，中央的口吃允许与大多数I类和III类病毒融合蛋白的可用结构进行紧密的结构比对。该结构进一步显示了许多稳定融合后发夹构象的链内盐桥，其中之一涉及天冬氨酸，该天冬氨酸似乎在低pH活化后从与稳定信号肽的关键相互作用中释放出来。

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  • 作者

    Sebastien lgonet; Marie-Christine Vaney; Clemens Vonhrein; Gerard Bricogne; Enrico A. Stura; Hans Hengartner; Bruno Eschli; Felix A. Rey;

  • 作者单位

    Departement de Virologie, Unite de Virologie Structurale, Institut Pasteur, F-75724 Paris Cedex 15, France,Centre National de la Recherche Scientifique,Unite de Recherche Associee 3015, F-75724 Paris Cedex 15, France;

    Departement de Virologie, Unite de Virologie Structurale, Institut Pasteur, F-75724 Paris Cedex 15, France,Centre National de la Recherche Scientifique,Unite de Recherche Associee 3015, F-75724 Paris Cedex 15, France;

    Global Phasing Ltd., Cambridge CB3 OAX, United Kingdom;

    Global Phasing Ltd., Cambridge CB3 OAX, United Kingdom;

    Commissariat a I'Energie Atomique Saclay, Service d'lngenierie et d'Etudes des Proteines, CE-Saclay F-91191 Gif-sur-Yvette Cedex, France;

    Institute of Experimental Immunology, University Hospital, CH 8557 Zurich, Switzerland;

    Institute of Experimental Immunology, University Hospital, CH 8557 Zurich, Switzerland;

    Departement de Virologie, Unite de Virologie Structurale, Institut Pasteur, F-75724 Paris Cedex 15, France,Centre National de la Recherche Scientifique,Unite de Recherche Associee 3015, F-75724 Paris Cedex 15, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    enveloped viruses; membrane fusion; RNA viruses; emerging viruses;

    机译：包膜病毒;膜融合RNA病毒;新兴病毒;

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