...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Pan-genome of the dominant human gut-associated archaeon, Methanobrevibacter smithii, studied in twins
【24h】

Pan-genome of the dominant human gut-associated archaeon, Methanobrevibacter smithii, studied in twins

机译：在双胞胎中研究了占主导地位的人类肠道相关古细菌的泛基因组

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The human gut microbiota harbors three main groups of H2-con-suming microbes: methanogens induding the dominant archaeon, Methanobrevibacter smithii, a polyphyletic group of acetogens, and sulfate-reducing bacteria. Defining their roles in the gut is important for understanding how hydrogen metabolism affects the efficiency of fermentation of dietary components. We quantified methanogens in fecal samples from 40 healthy adult female mono-zygotic (MZ) and 28 dizygotic (DZ) twin pairs, analyzed bacterial 16S rRNA datasets generated from their fecal samples to identify taxa that co-occur with methanogens, sequenced the genomes of 20 M. smithii strains isolated from families of MZ and DZ twins, and performed RNA-Seq of a subset of strains to identify their responses to varied formate concentrations. The concordance rate for methanogen carriage was significantly higher for MZ versus DZ twin pairs. Co-occurrence analysis revealed 22 bacterial species-level taxa positively correlated with methanogens: all but two were members of the Clostridiales, with several being, or related to, known hydrogen-producing and -consuming bacteria. The M. smithii pan-genome contains 987 genes conserved in all strains, and 1,860 variably represented genes. Strains from MZ and DZ twin pairs had a similar degree of shared genes and SNPs, and were significantly more similar than strains isolated from mothers or members of other families. The 101 adhesin-like proteins (ALPs) in the pan-genome (45 ± 6 per strain) exhibit strain-specific differences in expression and responsiveness to formate. We hypothesize that M. smithii strains use their different repertoires of ALPs to create diversity in their metabolic niches, by allowing them to establish syntrophic relationships with bacterial partners with differing metabolic capabilities and patterns of co-occurrence.
机译：人类肠道菌群包含三个主要的食用H2的微生物：产自优势古细菌的产甲烷菌，史密斯短杆菌，产乙酸菌的多系群和减少硫酸盐的细菌。定义它们在肠道中的作用对于理解氢代谢如何影响饮食成分的发酵效率非常重要。我们对40个健康成年雌性单卵双胎（MZ）和28个双卵双胎（DZ）双胞胎对的粪便样本中的产甲烷菌进行了定量分析，分析了从其粪便样本中产生的细菌16S rRNA数据集，以鉴定与产甲烷菌共生的类群，并测序了从MZ和DZ双胞胎家族中分离出20个史密斯猪笼草菌株，并对一部分菌株进行RNA-Seq鉴定，以确定它们对各种甲酸浓度的反应。 MZ与DZ双胞胎对的产甲烷菌运输一致性率显着更高。共现分析揭示了22种与产甲烷菌呈正相关的细菌类群：除2种外，其余均为梭菌的成员，其中几种与已知的产氢和耗氢细菌有关，或与之相关。铁匠史密斯泛基因组包含所有菌株中保守的987个基因，以及1,860个可变代表的基因。来自MZ和DZ双胞胎对的菌株具有相似程度的共享基因和SNP，并且比从母亲或其他家庭成员中分离出的菌株相似得多。泛基因组中的101种粘附素样蛋白（ALPs）（每个菌株45±6）在表达和对甲酸盐的响应性方面表现出菌株特异性差异。我们假设M. smithii菌株通过允许它们与具有不同代谢能力和共现模式的细菌伴侣建立同养关系，从而利用其不同的ALP种类在其代谢生态位中创造多样性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号