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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Electrokinesis is a microbial behavior that requires extracellular electron transport
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Electrokinesis is a microbial behavior that requires extracellular electron transport

机译：电动运动是一种微生物行为，需要细胞外电子传输

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摘要

We report a previously undescribed bacterial behavior termed electrokinesis. This behavior was initially observed as a dramatic increase in cell swimming speed during reduction of solid MnO_2 particles by the dissimilatory metal-reducing bacterium Shewanella oneidensis MR-1. The same behavioral response was observed when cells were exposed to small positive applied potentials at the working electrode of a microelectrochemical cell and could be tuned by adjusting the potential on the working electrode. Electro-kinesis was found to be different from both chemotaxis and galva-notaxis but was absent in mutants defective in electron transport to solid metal oxides. Using in situ video microscopy and cell tracking algorithms, we have quantified the response for different strains of Shewanella and shown that the response correlates with current-generating capacity in microbial fuel cells. The electrokinetic response was only exhibited by a subpopulation of cells closest to the MnO_2 particles or electrodes. In contrast, the addition of 1 mM 9,10-anthraquinone-2,6-disulfonic acid, a soluble electron shuttle, led to increases in motility in the entire population. Electrokinesis is defined as a behavioral response that requires functional extracellular electron transport and that is observed as an increase in cell swimming speeds and lengthened paths of motion that occur in the proximity of a redox active mineral surface or the working electrode of an electrochemical cell.
机译：我们报告了以前未描述的细菌行为，称为电动运动。最初观察到此行为是在异化金属还原细菌沙伊氏菌（Shewanella oneidensis）MR-1还原固体MnO_2颗粒过程中细胞游泳速度的急剧增加。当细胞在微电化学电池的工作电极上暴露于小的正施加电势时，可以观察到相同的行为响应，并且可以通过调节工作电极上的电势来对其进行调节。发现电动力学不同于趋化性和电刺激性，但在电子向固体金属氧化物的电子传输缺陷的突变体中不存在。使用原位视频显微镜和细胞跟踪算法，我们已经量化了希瓦氏菌不同菌株的响应，并表明该响应与微生物燃料电池中的发电能力相关。仅通过最接近MnO_2颗粒或电极的细胞亚群表现出电动反应。相反，添加1 mM 9,10-蒽醌-2,6-二磺酸（一种可溶的电子穿梭物）导致整个人群的运动性增加。电动运动被定义为需要功能性细胞外电子传输的行为反应，并且被观察为在氧化还原活性矿物表面或电化学电池的工作电极附近发生的细胞游泳速度的增加和运动路径的延长。

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  • 作者

    H. W. Harris; M. Y. El-Naggar; O. Bretschger; M. J. Ward; M. F. Romine; A. Y. Obraztsova; K. H. Nealson;

  • 作者单位

    Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089;

    Department of Physics and Astronomy, University of Southern California, Los Angeles, CA 90089;

    J. Craig Venter Institute, San Diego, CA 92121;

    Department of Earth Sciences, University of Southern California, Los Angeles, CA 90089;

    Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352;

    Department of Earth Sciences, University of Southern California, Los Angeles, CA 90089;

    Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089 J. Craig Venter Institute, San Diego, CA 92121 Department of Earth Sciences, University of Southern California, Los Angeles, CA 90089;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    bacteria; microbial fuel cells; motility; chemotaxis; galvanotaxis;

    机译：菌;微生物燃料电池动力趋化性通电;

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