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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The γ isoform of CaM kinase II controls mouse egg activation by regulating cell cycle resumption
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The γ isoform of CaM kinase II controls mouse egg activation by regulating cell cycle resumption

机译：CaM激酶II的γ亚型通过调节细胞周期的恢复来控制​​小鼠卵的活化

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摘要

Fertilization triggers a rise in intracellular Ca~(2+) concentration ([Ca~(2+)]_i) in the egg that initiates a series of events known as egg activation. These events include cortical granule exocytosis that establishes a block to polyspermy, resumption of meiosis, and recruitment of maternal mRNAs into polysomes for translation. Several calcium-dependent proteins, including calcium/calmodulin-dependent protein kinase II (CaMKII), have been implicated in egg activation. However, the precise role of CaMKII in mediating specific events of egg activation and the identity of the isoform(s) present in mouse eggs have not been unequivocally established. Through targeted deletion of the γ isoform of CaMKII, we find that CaMKIIy is the predominant CaMKII isoform in mouse eggs and that it is essential for egg activation. Although CaMKII_γ~(-/-) eggs exhibit a normal pattern of Ca~(2+) oscillations after insemination and undergo cortical granule exocytosis, they fail to resume meiosis or to recruit maternal mRNAs. Surprisingly, we find that the recruitment of maternal mRNAs does not directly depend on CaMKII, but requires elevated [Ca~(2+)]_i and metaphase II exit. We conclude that CaMKIIy specifically controls mouse egg activation by regulating cell cycle resumption.
机译：受精会触发卵中细胞内Ca〜（2+）浓度（[Ca〜（2 +）] _ i）的升高，从而引发一系列称为卵活化的事件。这些事件包括对多精子形成阻滞的皮质颗粒胞吐作用，减数分裂的恢复以及将母体mRNA募集到多核糖体中进行翻译。几种钙依赖性蛋白，包括钙/钙调蛋白依赖性蛋白激酶II（CaMKII），与卵的活化有关。但是，尚未明确确定CaMKII在介导卵活化的特定事件中的确切作用以及在小鼠卵中存在的同工型的身份。通过有针对性地删除CaMKII的γ亚型，我们发现CaMKIIy是小鼠卵子中主要的CaMKII异构体，并且它对卵子的激活至关重要。尽管CaMKII_γ〜（-/-）卵在受精后表现出正常的Ca〜（2+）振荡模式并经历皮质颗粒胞吐作用，但它们无法恢复减数分裂或募集母体mRNA。令人惊讶地，我们发现母体mRNA的募集并不直接取决于CaMKII，而是需要升高的[Ca〜（2 +）] _ i和中期II退出。我们得出结论，CaMKIIy通过调节细胞周期的恢复来特异性地控制小鼠卵的活化。

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    Johannes Backs; Paula Stein; Thea Backs; Francesca E. Duncan; Chad E. Grueter; John McAnally; Xiaoxia Qi; Richard M. Schultz; Eric N. Olson;

  • 作者单位

    Department of Cardiology, University of Heidelberg, 69120 Heidelberg, Germany Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9148;

    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104-6018;

    Department of Cardiology, University of Heidelberg, 69120 Heidelberg, Germany Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9148;

    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104-6018;

    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9148;

    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9148;

    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9148;

    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104-6018;

    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9148;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    CaMKII; fertilization; metaphase II exit;

    机译：CaMKII;受精中期II出口;

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