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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade
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TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade

机译：TRPM1是mGluR6级联中视网膜ON双极细胞转导通道的组成部分

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摘要

An essential step in intricate visual processing is the segregation of visual signals into ON and OFF pathways by retinal bipolar cells (BCs). Glutamate released from photoreceptors modulates the photoresponse of ON BCs via metabotropic glutamate receptor 6 (mGluR6) and G protein (Go) that regulates a cation channel. However, the cation channel has not yet been unequivocally identified. Here, we report a mouse TRPM1 long form (TRPM1-L) as the cation channel. We found that TRPM1-L localization is developmentally restricted to the dendritic tips of ON BCs in colocalization with mGluR6. TRPM1 null mutant mice completely lose the photoresponse of ON BCs but not that of OFF BCs. In the TRPM1-L-expressing cells, TRPM1-L functions as a constitutively active nonselective cation channel and its activity is negatively regulated by Go in the mGluR6 cascade. These results demonstrate that TRPM1-L is a component of the ON BC transduction channel downstream of mGluR6 in ON BCs.
机译：复杂的视觉处理的重要步骤是通过视网膜双极细胞（BCs）将视觉信号分离为ON和OFF通路。从感光器释放的谷氨酸盐通过代谢型谷氨酸受体6（mGluR6）和调节阳离子通道的G蛋白（Go）调节ON BC的光响应。但是，阳离子通道尚未明确鉴定。在这里，我们报告了鼠标TRPM1长形式（TRPM1-L）作为阳离子通道。我们发现在与mGluR6共定位中，TRPM1-L定位在发展上仅限于ON BCs的树突尖端。 TRPM1空突变小鼠完全失去ON BCs的光响应，但没有失去OFF BCs的光响应。在表达TRPM1-L的细胞中，TRPM1-L充当组成型活性非选择性阳离子通道，其活性受到mGluR6级联反应中Go的负调控。这些结果表明，TRPM1-L是ON BC中mGluR6下游ON BC转导通道的组成部分。

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  • 作者

    Chieko Koike; Takehisa Obara; Yoshitsugu Uriu; Tomohiro Numata; Rikako Sanuki; Kentarou Miyata; Toshiyuki Koyasu; Shinji Ueno; Kazuo Funabiki; Akiko Tani; Hiroshi Ueda; Mineo Kondo; Yasuo Mori; Masao Tachibana; Takahisa Furukawa;

  • 作者单位

    Departments of Developmental Biology Osaka Bioscience Institute, Osaka 565-0874, Japan;

    Department of Psychology, Graduate School of Humanities and Sociology, University of Tokyo, Tokyo 113-0033, Japan;

    Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan;

    Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan;

    Departments of Developmental Biology Osaka Bioscience Institute, Osaka 565-0874, Japan;

    Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan;

    Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan;

    Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan;

    Departments of Systems Biology, Osaka Bioscience Institute, Osaka 565-0874, Japan;

    Departments of Developmental Biology Osaka Bioscience Institute, Osaka 565-0874, Japan;

    Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu 501-1193, Japan;

    Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan;

    Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan;

    Department of Psychology, Graduate School of Humanities and Sociology, University of Tokyo, Tokyo 113-0033, Japan;

    Departments of Developmental Biology Osaka Bioscience Institute, Osaka 565-0874, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    G protein; retina; photoresponse; glutamate; vision;

    机译：G蛋白;视网膜光响应谷氨酸视力;

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