...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Label-free detection of protein-protein interactions using a calmodulin-modified nanowire transistor
【24h】

Label-free detection of protein-protein interactions using a calmodulin-modified nanowire transistor

机译：使用钙调蛋白修饰的纳米线晶体管对蛋白质间相互作用进行无标记检测

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, we describe a highly sensitive and reusable silicon nanowire field-effect transistor for the detection of protein-protein interactions. This reusable device was made possible by the reversible association of glutathione S-transferase-tagged calmodulin with a glutathione modified transistor. The calmodulin-modified transistor exhibited selective electrical responses to Ca~(2+) (≥1 μM) and purified cardiac troponin I (～7 nM); the change in conductivity displayed a linear dependence on the concentration of troponin I in a range from 10 nM to 1 μM. These results are consistent with the previously reported concentration range in which the dissociation constant for the troponin I-calmodulin complex was determined. The minimum concentration of Ca~(2+) required to activate calmodulin was determined to be 1 μM. We have also successfully demonstrated that the N-type Ca~(2+) channels, expressed by cultured 293T cells, can be recognized specifically by the calmodulin-modified nanowire transistor. This sensitive nanowire transistor can serve as a high-throughput biosensor and can also substitute for immu-noprecipitation methods used in the identification of interacting proteins.
机译：在这项研究中，我们描述了一种高度敏感和可重复使用的硅纳米线场效应晶体管，用于检测蛋白质-蛋白质相互作用。通过谷胱甘肽S-转移酶标记的钙调蛋白与谷胱甘肽修饰的晶体管的可逆结合，使这种可重复使用的装置成为可能。钙调蛋白修饰的晶体管对Ca〜（2+）（≥1μM）和纯化的心肌肌钙蛋白I（〜7 nM）表现出选择性电响应；电导率的变化在10 nM至1μM范围内显示出对肌钙蛋白I浓度的线性依赖性。这些结果与先前报道的浓度范围一致，在该浓度范围内确定了肌钙蛋白I-钙调蛋白复合物的解离常数。确定激活钙调蛋白所需的Ca〜（2+）的最低浓度为1μM。我们还成功地证明了钙调蛋白修饰的纳米线晶体管可以特异性识别由培养的293T细胞表达的N型Ca〜（2+）通道。这种灵敏的纳米线晶体管可以用作高通量生物传感器，也可以替代用于鉴定相互作用蛋白的免疫沉淀方法。

著录项

  • 来源
  • 作者

    Tsung-Wu Lin; Po-Jen Hsieh; Chih-Lung Lin; Yi-Ya Fang; Jia-Xun Yang; Chia-Chang Tsai; Pei-Ling Chiang; Chien-Yuan Pan; Yit-Tsong Chen;

  • 作者单位

    Institute of Atomic and Molecular Sciences, Academia Sinica, P.O. Box 23-166, Taipei 106, Taiwan Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan;

    Institute of Atomic and Molecular Sciences, Academia Sinica, P.O. Box 23-166, Taipei 106, Taiwan Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan;

    Institute of Zoology and Department of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan;

    Institute of Zoology and Department of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan;

    Institute of Atomic and Molecular Sciences, Academia Sinica, P.O. Box 23-166, Taipei 106, Taiwan Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan;

    Institute of Atomic and Molecular Sciences, Academia Sinica, P.O. Box 23-166, Taipei 106, Taiwan Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan;

    Institute of Atomic and Molecular Sciences, Academia Sinica, P.O. Box 23-166, Taipei 106, Taiwan Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan;

    Institute of Zoology and Department of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan;

    Institute of Atomic and Molecular Sciences, Academia Sinica, P.O. Box 23-166, Taipei 106, Taiwan Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    calcium ion; glutathione S-transfrease; N-type calcium channel; silicon nanowire field-effect transistor;

    机译：钙离子谷胱甘肽S-转移酶;N型钙通道;硅纳米线场效应晶体管;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号