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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans
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Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans

机译:秀丽隐杆线虫的精子发生特异性26G-RNA和耐热精子需要Argonautes ALG-3和ALG-4

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摘要

Gametogenesis is a thermosensitive process in numerous metazo-ans, ranging from worms to man. In Caenorhabditis elegans, a variety of RNA-binding proteins that associate with germ-line nuage (P granules), including the Piwi-clade argonaute PRG-1, have been implicated in maintaining fertility at elevated temperature. Here we describe the role of two AGO-class paralogs, alg-3 (T22B3.2) and alg-4 (ZK757.3), in promoting thermotolerant male fertility. A rescuing GFP::alg-3 transgene is localized to P granules beginning at the late pachytene stage of male gametogenesis. alg-3/4 double mutants lack a subgroup of small RNAs, the 26G-RNAs which target and appear to down-regulate numerous spermatogenesis-expressed mRNAs. These findings add to a growing number of AGO pathways required for thermotolerant fertility in C elegans and support a model in which AGOs and their small RNA cofactors function to promote robustness in gene-expression networks.
机译:在从蠕虫到人的众多后生动物中,配子发生是一个热敏过程。在秀丽隐杆线虫中,涉及与生殖系成核(P颗粒)相关的各种RNA结合蛋白,包括长毛Pi皮PRG-1,在维持高温下的育性中都有牵连。在这里,我们描述了两个AGO类旁系同源物alg-3(T22B3.2)和alg-4(ZK757.3)在促进耐热男性生育能力中的作用。抢救的GFP :: alg-3转基因位于雄性配子体的后期粗线期开始的P颗粒上。 alg-3 / 4双突变体缺少小RNA的亚组,即靶向并似乎下调许多精子生成表达的mRNA的26G-RNA。这些发现增加了秀丽隐杆线虫耐热生育所需要的越来越多的AGO途径,并支持了其中AGO及其小RNA辅因子起作用以促进基因表达网络稳健性的模型。

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  • 作者单位

    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606;

    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606 Gulbenkian PhD Programme in Biomedicine, Rua da Quinta Grande, 6, 2780-156 Oeiras, Portugal;

    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606;

    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606 The Howard Hughes Medical Institute,University of Massachusetts Medical School, Worcester, MA 01606;

    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606 Instituto de Medicina Molecular, Faculdade de Medicina,Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisbon, Portugal;

    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606;

    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606 The Howard Hughes Medical Institute,University of Massachusetts Medical School, Worcester, MA 01606;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    gene expression; RNAi; germline; P granules; spermiogenesis;

    机译:基因表达;RNAi;种系P颗粒;精子发生;

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