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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The PPH1 phosphatase is specifically involved in LHCII dephosphorylation and state transitions in Arabidopsis
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The PPH1 phosphatase is specifically involved in LHCII dephosphorylation and state transitions in Arabidopsis

机译：PPH1磷酸酶专门参与拟南芥中LHCII的去磷酸化和状态转变

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摘要

The ability of plants to adapt to changing light conditions depends on a protein kinase network in the chloroplast that leads to the reversible phosphorylation of key proteins in the photosynthetic membrane. Phosphorylation regulates, in a process called state transition, a profound reorganization of the electron transfer chain and remodeling of the thylakoid membranes. Phosphorylation governs the association of the mobile part of the light-harvesting antenna LHCII with either photosystem I or photosystem II. Recent work has identified the redox-regulated protein kinase STN7 as a major actor in state transitions, but the nature of the corresponding phospha-tases remained unknown. Here we identify a phosphatase of Arabidopsis thaliana, called PPH1, which is specifically required for the dephosphorylation of light-harvesting complex II (LHCII). We show that this single phosphatase is largely responsible for the dephosphorylation of Lhcb1 and Lhcb2 but not of the photosystem II core proteins. PPH1, which belongs to the family of monomeric PP2C type phosphatases, is a chloroplast protein and is mainly associated with the stroma lamellae of the thylakoid membranes. We demonstrate that loss of PPH1 leads to an increase in the antenna size of photosystem I and to a strong impairment of state transitions. Thus phosphorylation and dephosphorylation of LHCII appear to be specifically mediated by the kinase/phosphatase pair STN7 and PPH1. These two proteins emerge as key players in the adaptation of the photosynthetic apparatus to changes in light quality and quantity.
机译：植物适应变化的光照条件的能力取决于叶绿体中的蛋白激酶网络，该蛋白激酶网络可导致光合膜中关键蛋白的可逆磷酸化。磷酸化以状态转移的过程调节电子转移链的深刻重组和类囊体膜的重塑。磷酸化控制光捕获天线LHCII的移动部分与光电系统I或光电系统II的关联。最近的工作已确定氧化还原调节的蛋白激酶STN7是状态转换的主要参与者，但相应的磷酸酶的性质仍然未知。在这里，我们确定了拟南芥的一种磷酸酶，称为PPH1，这对于光捕获复合物II（LHCII）的去磷酸化是特别需要的。我们表明，这种单一的磷酸酶在很大程度上负责Lhcb1和Lhcb2的去磷酸化，而不是光系统II核心蛋白的去磷酸化。 PPH1属于单体PP2C型磷酸酶家族，是一种叶绿体蛋白，主要与类囊体膜的基质层相关。我们证明PPH1的丢失会导致光系统I的天线尺寸增加，并严重损害状态转换。因此，LHCII的磷酸化和去磷酸化似乎是由激酶/磷酸酶对STN7和PPH1特异性介导的。这两种蛋白质成为光合装置适应光质和光量变化的关键参与者。

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