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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A link between the assembly of flagella and lipooligosaccharide of the Gram-negative bacterium Campylobacter jejuni
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A link between the assembly of flagella and lipooligosaccharide of the Gram-negative bacterium Campylobacter jejuni

机译：空肠弯曲菌空肠弯曲杆菌鞭毛和脂寡糖的装配之间的联系

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摘要

Campylobacter jejuni is the leading cause of acute bacterial diarrhea worldwide and is implicated in development of Guillain-Barre syndrome. Two major surface features, the outer membrane lipooligosaccharide and flagella, are highly variable and are often targets for modification. Presumably, these modifications provide a competitive advantage to the bacterium. In this work, we identify a gene encoding a phosphoethanolamine (pEtN) transferase (Cj0256) that serves a dual role in modifying not only the lipooligosaccharide lipid anchor lipid A with pEtN, but also the flagellar rod protein FlgG. Generation of a mutant in C jejuni 81-176 by interruption of cj0256 resulted in the absence of pEtN modifications on lipid A as well as FlgG. The cj0256 mutant showed a 20-fold increase in sensitivity to the cationic antimicrobial peptide, polymyxin B, as well as a decrease in motility. Transmission EM of the cy0256 mutant revealed a population (approximately 95%) lacking flagella, indicating that, without pEtN modification of FlgG, flagella production is hindered. Most intriguing, this research identifies a pEtN transferase showing preference for two periplasmic substrates linking membrane biogenesis and flagellar assembly. Cj0256 is a member of a large family of mostly uncharacterized proteins that may play a larger role in the decoration of bacterial surface structures.
机译：空肠弯曲杆菌是全世界急性细菌性腹泻的主要原因，并与格林-巴利综合征的发展有关。外膜脂寡糖和鞭毛这两个主要表面特征变化很大，经常成为修饰的目标。据推测，这些修饰为细菌提供了竞争优势。在这项工作中，我们确定了编码磷酸乙醇胺（pEtN）转移酶（Cj0256）的基因，该基因不仅可以用pEtN修饰脂寡糖脂质锚定脂质A，而且还可以修饰鞭毛杆蛋白FlgG。通过中断cj0256而在空肠弯曲杆菌81-176中产生突变体导致在脂质A和FlgG上不存在pEtN修饰。 cj0256突变体显示出对阳离子抗微生物肽多粘菌素B的敏感性提高了20倍，并且运动性降低。 cy0256突变体的传播EM显示缺乏鞭毛的种群（约95％），表明如果不对FlgG进行pEtN修饰，鞭毛的产生就会受到阻碍。最引人入胜的是，这项研究确定了pEtN转移酶，该酶显示出对连接膜生物发生和鞭毛组装的两种周质底物的偏爱。 Cj0256是大部分未鉴定蛋白的大家族的成员，这些蛋白可能在细菌表面结构的修饰中发挥更大的作用。

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