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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Cell pole-specific activation of a critical bacterial cell cycle kinase
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Cell pole-specific activation of a critical bacterial cell cycle kinase

机译：关键细菌细胞周期激酶的细胞极特异性激活

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摘要

Caulobacter crescentus integrates phospho-signaling pathways and transcription factor regulatory cascades to drive the cell cycle. Despite the essential role of the CckA histidine kinase in the control of cell cycle events, the factors that signal its activation at a specific time in the cell cycle have remained elusive. A conditional genetic screen for CckA mislocalization mutants, using automated fluorescence microscopy and an image processing platform, revealed that the essential DivL protein kinase promotes CckA localization, autophosphorylation, and activity at the new cell pole. The transient accumulation of DivL at the new cell pole, but not its kinase activity, is required for the localization and activation of CckA. Because DivL and CckA accumulate at the same cell pole after the initiation of DNA replication and were found to interact in vivo, we propose that DivL recruits CckA to the pole, thereby promoting its autophosphorylation and activity.
机译：新月形杆菌整合了磷酸信号通路和转录因子调节级联反应，以驱动细胞周期。尽管CckA组氨酸激酶在控制细胞周期事件中起着至关重要的作用，但在细胞周期特定时间发出信号激活其的因素仍然难以捉摸。使用自动荧光显微镜和图像处理平台进行的CckA定位错误突变体的条件遗传筛选显示，必需的DivL蛋白激酶可促进CckA定位，自磷酸化和在新细胞极的活性。 DivL在新的细胞极处的瞬时积累，而不是其激酶活性，对于CckA的定位和激活是必需的。由于DivL和CckA在DNA复制开始后聚集在同一细胞极，并发现在体内相互作用，因此我们建议DivL将CckA募集到该极，从而促进其自磷酸化和活性。

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  • 作者

    Antonio A. Iniesta; Nathan J. Hillson; Lucy Shapiro;

  • 作者单位

    Department of Developmental Biology, Beckman Center, Stanford University School of Medicine, Stanford, CA 94305;

    Department of Developmental Biology, Beckman Center, Stanford University School of Medicine, Stanford, CA 94305 Fuels Synthesis Division, Joint BioEnergy Institute, Emeryville, CA 94608;

    Department of Developmental Biology, Beckman Center, Stanford University School of Medicine, Stanford, CA 94305;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    caulobacter; CckA; DivL; polar localization;

    机译：杆菌CckA;DivL;极地定位;

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