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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Identification of neuronal subpopulations that project from hypothalamus to both liver and adipose tissue polysynaptically
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Identification of neuronal subpopulations that project from hypothalamus to both liver and adipose tissue polysynaptically

机译:鉴定从下丘脑突触到肝和脂肪组织的神经元亚群

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摘要

The autonomic nervous system regulates fuel availability and energy storage in the liver, adipose tissue, and other organs; however, the molecular components of this neural circuit are poorly understood. We sought to identify neural populations that project from the CNS indirectly through multisynaptic pathways to liver and epididymal white fat in mice using pseudorabies virus strains expressing different reporters together with BAC transgenesis and immunohistochemistry. Neurons common to both circuits were identified in subpopulations of the paraventricular nucleus of the hypothalamus (PVH) by double labeling with markers expressed in viruses injected in both sites. The lateral hypothalamus and arcuate nucleus of the hypothalamus and brainstem regions (nucleus of the solitary tract and A5 region) also project to both tissues but are labeled at later times. Connections from these same sites to the PVH were evident after direct injection of virus into the PVH, suggesting that these regions lie upstream of the PVH in a common pathway to liver and adipose tissue (two metabolically active organs). These common populations of brainstem and hypothalamic neurons express neuropeptide Y and proopiomelanocortin in the arcuate nucleus, melanin-concentrating hormone, and orexin in the lateral hypothalamus and in the corticotrophin-releasing hormone and oxytocin in the PVH. The delineation of this circuitry will facilitate a functional analysis of the possible role of these potential command-like neurons to modulate autonomic outflow and coordinate metabolic responses in liver and adipose tissue.
机译:自主神经系统调节肝脏,脂肪组织和其他器官中的燃料供应和能量存储;然而,对该神经回路的分子成分了解得很少。我们力图使用表达不同报告基因的假狂犬病毒株以及BAC转基因和免疫组化方法,鉴定出通过多突触途径间接从中枢神经系统投射到肝脏和附睾白色脂肪的神经种群。通过用在两个部位注射的病毒中表达的标志物进行双重标记,在下丘脑室旁核(PVH)的亚群中鉴定出两个回路共有的神经元。下丘脑的外侧下丘脑和弓形核以及脑干区域(孤立道和A5区域的核)也投射到两个组织,但在以后被标记。直接将病毒注入PVH后,这些相同部位与PVH的连接就很明显,这表明这些区域位于PVH的上游,是通向肝脏和脂肪组织(两个代谢活性器官)的共同途径。这些常见的脑干和下丘脑神经元群体在弓形核,下丘脑外侧,黑色素浓缩激素和皮质激素释放激素,催产素和催产素中表达神经肽Y和proopiomelanocortin。该电路的描绘将有助于对这些潜在的命令状神经元可能的功能进行功能分析,以调节自主神经流出并协调肝脏和脂肪组织中的代谢反应。

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  • 作者单位

    Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10065;

    Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10065 Merck Research Laboratories, Railway, NJ 07065;

    Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10065 Department of Pathology, Weill Cornell Medical College, New York, NY 10065;

    Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10065;

    Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10065 Genomics Core Laboratory, Memorial Sloan Kettering Hospital, New York, NY 10065;

    Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10065 Renovis, San Fransisco, CA 94080;

    Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10065;

    Department of Pharmacology, University of Cambridge, Cambridge, CB2 1PD United Kingdom;

    Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10065 Howard Hughes Medical Institute, Rockefeller University, New York, NY 10065;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    autonomic nervous system; neuronal tracing; pseudorabies virus;

    机译:自主神经系统;神经元追踪;伪狂犬病病毒;

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