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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Transient tether between the SRP RNA and SRP receptor ensures efficient cargo delivery during cotranslational protein targeting
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Transient tether between the SRP RNA and SRP receptor ensures efficient cargo delivery during cotranslational protein targeting

机译：SRP RNA和SRP受体之间的瞬时系链可确保在共翻译蛋白靶向过程中有效地运送货物

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摘要

Kinetic control of macromolecular interactions plays key roles in biological regulation. An example of such control occurs in cotranslational protein targeting by the signal recognition particle (SRP), during which the SRP RNA and the cargo both accelerate complex assembly between the SRP and SRP receptor FtsY 10~2-fold. The molecular mechanism underlying these rate accelerations was unclear. Here we show that a highly conserved basic residue, Lys399, on the lateral surface of FtsY provides a novel RNA tetraloop receptor to mediate the SRP RNA- and cargo-induced acceleration of SRP-FtsY complex assembly. We propose that the SRP RNA, by using its tetraloop to interact with FtsY-Lys399, provides a transient tether to stabilize the early stage and transition state of complex formation; this accelerates the assembly of a stable SRP-FtsY complex and allows the loading of cargo to be efficiently coupled to its membrane delivery. The use of a transient tether to increase the lifetime of collisional intermediates and reduce the dimension of diffusional search represents a novel and effective mechanism to accelerate macromolecular interactions.
机译：大分子相互作用的动力学控制在生物调节中起关键作用。这种控制的一个例子发生在信号识别颗粒（SRP）的共翻译蛋白靶向中，在此期间SRP RNA和货物都加速了SRP和SRP受体FtsY之间10〜2倍的复杂组装。这些速率加速的基础分子机制尚不清楚。在这里，我们显示FtsY侧表面上高度保守的碱性残基Lys399提供了一种新颖的RNA四环受体来介导SRP RNA和货物诱导的SRP-FtsY复杂装配的加速。我们建议SRP RNA，通过使用其四环与FtsY-Lys399相互作用，提供了一个短暂的系链，以稳定复合物形成的早期阶段和过渡状态。这加速了稳定的SRP-FtsY复合物的组装，并使货物的装载有效地与其膜传递结合。使用瞬态系链以延长碰撞中间体的寿命并减小扩散搜索的范围代表了一种新颖且有效的机制，可促进大分子相互作用。

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