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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >An ATPase promotes autophosphorylation of the pattern recognition receptor XA21 and inhibits XA21 -mediated immunity
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An ATPase promotes autophosphorylation of the pattern recognition receptor XA21 and inhibits XA21 -mediated immunity

机译:ATPase促进模式识别受体XA21的自磷酸化并抑制XA21介导的免疫

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摘要

Cell-surface pattern recognition receptors (PRRs) are key components of the innate immune response in animals and plants. These receptors typically carry or associate with non-RD kinases to control early events of innate immunity signaling. Despite their importance, the mode of regulation of PRRs is largely unknown. Here we show that the rice PRR, XA21, interacts with XA21 binding protein 24 (XB24), a previously undescribed ATPase. XB24 promotes autophosphorylation of XA21 through its ATPase activity. Rice lines silenced for Xb24 display enhanced XA21-mediated immunity, whereas rice lines overexpressing XB24 are compromised for immunity. XB24 ATPase enzyme activity is required for XB24 function. XA21 is degraded in the presence of the pathogen-associated molecular pattern Ax21 when XB24 is overexpressed. These results demonstrate a function for this large class of broadly conserved ATPases in PRR-mediated immunity.
机译:细胞表面模式识别受体(PRR)是动植物固有免疫反应的关键组成部分。这些受体通常携带或与非RD激酶结合,以控制先天免疫信号的早期事件。尽管具有重要意义,但对PRR的调节模式仍知之甚少。在这里,我们显示了水稻PRR XA21与XA21结合蛋白24(XB24)(先前未描述的ATPase)相互作用。 XB24通过其ATPase活性促进XA21的自磷酸化。 Xb24沉默的水稻系显示出增强的XA21介导的免疫力,而过表达XB24的水稻系的免疫力受到损害。 XB24功能需要XB24 ATPase酶活性。当XB24过表达时,XA21在病原体相关分子模式Ax21的存在下降解。这些结果证明了这一类广泛保守的ATPase在PRR介导的免疫中的功能。

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