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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Visualization of nitric oxide production in the mouse main olfactory bulb by a cell-trappable copper(II) fluorescent probe
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Visualization of nitric oxide production in the mouse main olfactory bulb by a cell-trappable copper(II) fluorescent probe

机译:通过细胞可捕获的铜(II)荧光探针可视化小鼠主嗅球中一氧化氮的产生

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摘要

We report the visualization of NO production using fluorescence in tissue slices of the mouse main olfactory bulb. This discovery was possible through the use of a novel, cell-trappable probe for intra-cellular nitric oxide detection based on a symmetric scaffold with two NO-reactive sites. Ester moieties installed onto the fluorescent probe are cleaved by intracellular esterases to yield the corresponding negatively charged, cell-impermeable acids. The trappable probe Cu_2(FL2E) and the membrane-impermeable acid derivative Cu_2(FL2A) respond rapidly and selectively to NO in buffers that simulate biological conditions, and application of Cu_2(FL2E) leads to detection of endogenously produced NO in cell cultures and olfactory bulb brain slices.
机译:我们报告了使用荧光在小鼠主要嗅球组织切片中产生NO的可视化。通过使用具有两个NO反应位点的对称支架的新型细胞可插入探针进行细胞内一氧化氮检测,这一发现是可能的。安装在荧光探针上的酯部分被细胞内酯酶切割,产生相应的带负电荷的,细胞不可渗透的酸。可捕获的探针Cu_2(FL2E)和不可渗透膜的酸衍生物Cu_2(FL2A)在模拟生物学条件的缓冲液中对NO产生快速选择性的响应,而Cu_2(FL2E)的应用可检测细胞培养物中的内源性NO和嗅觉鳞茎脑片。

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