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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy
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Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy

机译：使用原子力显微镜对细菌磁性细胞器磁小体的可视化和结构分析

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摘要

The unique ability of magnetotactic bacteria to navigate along a geomagnetic field is accomplished with the help of prokaryotic organelles, magnetosomes. The magnetosomes have well-ordered chain-like structures, comprising membrane-enveloped, nano-sized magnetic crystals, and various types of specifically associated proteins. In this study, we applied atomic force microscopy (AFM) to investigate the spatial configuration of isolated magnetosomes from Magnetospirillum magneticum AMB-1 in near-native buffer conditions. AFM observation revealed organic material with a ～7-nm thickness surrounding a magnetite crystal. Small globular proteins, identified as magnetosome-associated protein MamA, were distributed on the mica surface around the magnetosome. Immuno-labeling with AFM showed that MamA is located on the magnetosome surface. In vitro experiments showed that MamA proteins interact with each other and form a high molecular mass complex. These findings suggest that magnetosomes are covered with MamA oligomers in near-native environments. Furthermore, nanodissection revealed that magnetosomes are built with heterogeneous structures that comprise the organic layer. This study provides important clues to the supramolecular architecture of the bacterial organelle, the magnetosome, and insight into the function of the proteins localized in the organelle.
机译：趋磁细菌在地磁场中导航的独特能力是在原核细胞器磁小体的帮助下实现的。磁小体具有规则的链状结构，包括膜包裹的纳米级磁性晶体，以及各种类型的特异性缔合蛋白。在这项研究中，我们应用原子力显微镜（AFM）来研究在接近天然缓冲液条件下从Magnetospirillum magneticum AMB-1分离的磁小体的空间构型。原子力显微镜观察发现有机材料围绕磁铁矿晶体，厚度约为〜7 nm。小球状蛋白被鉴定为与磁小体相关的蛋白MamA，分布在磁小体周围的云母表面。用AFM免疫标记显示MamA位于磁小体表面。体外实验表明，MamA蛋白彼此相互作用并形成高分子复合物。这些发现表明，在近自然环境中，磁小体被​​MamA低聚物覆盖。此外，纳米解剖揭示了磁小体具有包含有机层的异质结构。这项研究为细菌细胞器，磁小体的超分子结构提供了重要线索，并深入了解了位于细胞器中的蛋白质的功能。

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    Daisuke Yamamoto; Azuma Taoka; Takayuki Uchihashi; Hideaki Sasaki; Hiroki Watanabe; Toshio Ando; Yoshihiro Fukumori;

  • 作者单位

    Department of Physics, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Sanban-cho, Chiyoda-ku, Tokyo 102-0075, Japan;

    rnDepartment of Life Science, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan;

    rnDepartment of Physics, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Sanban-cho, Chiyoda-ku, Tokyo 102-0075, Japan;

    rnDepartment of Life Science, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan;

    rnDepartment of Physics, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan;

    rnDepartment of Physics, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Sanban-cho, Chiyoda-ku, Tokyo 102-0075, Japan Frontier Science Organization, Kanazawa University, Kakuma-machi,Kanazawa 920-1192, Japan;

    rnDepartment of Life Science, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    magnetotactic bacteria; scanning probe microscopy; nanoparticle; TPR protein; protein localization;

    机译：趋磁细菌扫描探针显微镜纳米粒子TPR蛋白;蛋白质定位;

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