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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Ribosome recycling step in yeast cytoplasmic protein synthesis is catalyzed by eEF3 and ATP
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Ribosome recycling step in yeast cytoplasmic protein synthesis is catalyzed by eEF3 and ATP

机译:酵母胞质蛋白合成中的核糖体回收步骤被eEF3和ATP催化

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摘要

After each round of protein biosynthesis, the posttermination complex (PoTC) consisting of a ribosome, mRNA, and tRNA must be disassembled into its components for a new round of translation. Here, we show that a Saccharomyces cerevisiae model PoTC was disassembled by ATP and eukaryotic elongation factor 3 (eEF3). GTP or ITP functioned with less efficiency and adenosine 5γ'-(β,γ-imido)triphosphate did not function at all. The K_(cca), of eEF3 was 1.12 min~(-1). which is comparable to that of the in vitro initiation step. The disassembly reaction was inhibited by aminoglycosides and cycloheximide. The subunits formed from the yeast model PoTC remained separated under ionic conditions close to those existing in vivo, suggesting that they are ready to enter the initiation process. Based on our experimental techniques used in this paper, the release of mRNA and tRNA and ribosome dissociation took place simultaneously. No 40S·mRNA complex was observed, indicating that eEF3 action promotes ribosome recycling, not reinitiation.
机译:在每轮蛋白质生物合成之后,必须将由核糖体,mRNA和tRNA组成的后终止复合物(PoTC)分解成其成分,以进行新一轮的翻译。在这里,我们显示了酿酒酵母模型PoTC被ATP和真核生物延伸因子3(eEF3)分解。 GTP或ITP的效率较低,而腺苷5γ'-(β,γ-亚氨基)三磷酸根本不起作用。 eEF3的K_(cca)为1.12 min〜(-1)。与体外起始步骤相当。氨基糖苷和环己酰亚胺抑制了分解反应。由酵母模型PoTC形成的亚基在接近于体内存在的离子条件下仍保持分离状态,这表明它们已准备好进入启动过程。根据我们在本文中使用的实验技术,mRNA和tRNA的释放与核糖体解离同时发生。没有观察到40S·mRNA复合物,表明eEF3的作用促进了核糖体的再循环,而不是重新启动。

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