...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Direct regulation of phytoene synthase gene expression and carotenoid biosynthesis by phytochrome-interacting factors
【24h】

Direct regulation of phytoene synthase gene expression and carotenoid biosynthesis by phytochrome-interacting factors

机译：植物色素相互作用因子直接调控植物六烯合酶基因的表达和类胡萝卜素的生物合成

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Carotenoids are key for plants to optimize carbon fixing using the energy of sunlight. They contribute to light harvesting but also channel energy away from chlorophylls to protect the photosyn-thetic apparatus from excess light. Phytochrome-mediated light signals are major cues regulating carotenoid biosynthesis in plants, but we still lack fundamental knowledge on the components of this signaling pathway. Here we show that phytochrome-interacting factor 1 (PIF1) and other transcription factors of the phytochrome-interacting factor (PIF) family down-regulate the accumulation of carotenoids by specifically repressing the gene encoding phytoene synthase (PSY), the main rate-determining enzyme of the pathway. Both in vitro and in vivo evidence demonstrate that PIF1 directly binds to the promoter of the PSY gene, and that this binding results in repression of PSY expression. Light-triggered degradation of PIFs after interaction with photoactivated phytochromes during dee-tiolation results in a rapid derepression of PSY gene expression and a burst in the production of carotenoids in coordination with chlorophyll biosynthesis and chloroplast development for an optimal transition to photosynthetic metabolism. Our results also suggest a role for PIF1 and other PIFs in transducing light signals to regulate PSY gene expression and carotenoid accumulation during daily cycles of light and dark in mature plants.
机译：类胡萝卜素是植物利用阳光能量优化固碳的关键。它们有助于光的收集，但也可以将能量从叶绿素中带走，以保护光合装置免受过量的光侵害。植物色素介导的光信号是调节植物中类胡萝卜素生物合成的主要线索，但我们仍然缺乏有关该信号传导途径成分的基本知识。在这里我们表明，植物色素相互作用因子1（PIF1）和其他植物色素相互作用因子（PIF）的转录因子通过特异性抑制编码植物生长素合酶（PSY）的基因来下调类胡萝卜素的积累，这是主要的速率决定因素途径的酶。体外和体内的证据都表明，PIF1直接与PSY基因的启动子结合，并且这种结合导致PSY表达的抑制。在去黄化过程中与光活化的植物色素相互作用后，PIFs的光触发降解导致PSY基因表达快速降低，并且与叶绿素的生物合成和叶绿体的发育相协调的类胡萝卜素的产生爆发，从而实现了向光合代谢的最佳过渡。我们的研究结果还表明，PIF1和其他PIF在光信号转导过程中，在成熟植物的明暗周期中调节PSY基因表达和类胡萝卜素积累中发挥作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号