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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Ghrelin secretion stimulated by β_1-adrenergic receptors in cultured ghrelinoma cells and in fasted mice
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Ghrelin secretion stimulated by β_1-adrenergic receptors in cultured ghrelinoma cells and in fasted mice

机译：β_1-肾上腺素能受体刺激的生长激素释放瘤细胞和禁食小鼠的生长激素释放激素分泌

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摘要

Ghrelin, an octanoylated peptide hormone produced in the stomach, rises dramatically in mouse plasma during chronic severe calorie deprivation, an event that is essential to maintain life. The mechanism for this increase is not understood. Here, we study the control of ghrelin secretion in tissue culture cells delived from mice bearing ghrelinomas induced by a tissue-specific SV40 T-antigen transgene. We found that the ghrelin-secreting cells express high levels of mRNA encoding β_2-adrenergic receptors. Addition of norepinephrine or epi-nephrine to the culture medium stimulated ghrelin secretion, and this effect was blocked by atenolol, a selective pyadrenergic antagonist. When WT mice were treated with reserpine to deplete adrenergic neurotransmitters from sympathetic neurons, the fasting-induced increase in plasma ghrelin was blocked. Inhibition was also seen following atenolol administration. We conclude that ghrelin secretion during fasting is induced by adrenergic agents released by sympathetic neurons and acting directly on β_1 receptors on the ghrelin-secreting cells of the stomach.
机译：Ghrelin是胃中产生的一种辛酰化的肽激素，在长期严重的卡路里剥夺过程中，血浆中的Ghrelin急剧增加，这是维持生命所必需的。这种增加的机制尚不清楚。在这里，我们研究了由组织特异性SV40 T抗原转基因诱导的带有生长激素的小鼠的组织培养细胞中生长素释放肽分泌的控制。我们发现，生长素释放肽分泌细胞表达高水平的编码β_2-肾上腺素能受体的mRNA。向培养基中添加去甲肾上腺素或表肾上腺素可刺激生长素释放肽的分泌，而这种作用被选择性的肾上腺素拮抗剂阿替洛尔阻断。当用利血平治疗野生型小鼠以减少交感神经元的肾上腺素能神经递质时，空腹诱导的血浆生长素释放肽的增加被阻止。服用阿替洛尔后也观察到抑制作用。我们得出的结论是，禁食期间ghrelin的分泌是由交感神经元释放并直接作用于胃分泌ghrelin的细胞上的β_1受体的肾上腺素能引起的。

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  • 作者

    Tong-Jin Zhao; Ichiro Sakata; Robert Lin Li; Guosheng Liang; James A. Richardson; Michael S. Brown; Joseph L. Goldstein; Jeffrey M. Zigman;

  • 作者单位

    Departments of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390;

    rnDepartments of lnternal Medicine (Divisions of Hypothalamic Research and Endocrinology and Metabolism), University of Texas Southwestern Medical Center, Dallas, TX 75390;

    rnDepartments of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390;

    rnDepartments of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390;

    rnDepartments of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390;

    rnDepartments of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390;

    rnDepartments of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390;

    rnDepartments of lnternal Medicine (Divisions of Hypothalamic Research and Endocrinology and Metabolism), University of Texas Southwestern Medical Center, Dallas, TX 75390 Departments of Psychiatry, University of Texas Southwestern Medical Center, Dallas, TX 75390;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    endocrine tumors; PG-1 cells; atenolol; octanoate; SV40-T antigen;

    机译：内分泌肿瘤PG-1细胞;阿替洛尔辛酸SV40-T抗原;

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