首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >VIVID interacts with the WHITE COLLAR complex and FREQUENCY-interacting RNA helicase to alter light and clock responses in Neurospora
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VIVID interacts with the WHITE COLLAR complex and FREQUENCY-interacting RNA helicase to alter light and clock responses in Neurospora

机译:VIVID与WHITE COLLAR复合物和与FREQUENCY相互作用的RNA解旋酶相互作用,以改变Neurospora中的光和时钟响应

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摘要

The photoreceptor and PAS/LOV protein VIVID (WD) modulates blue-light signaling and influences light and temperature responses of the circadian clock in Neurospora crassa. One of the main actions of VVD on the circadian clock is to influence circadian clock phase by regulating levels of the transcripts encoded by the central clock gene frequency (frq). How this regulation is achieved is unknown. Here we show that WD interacts with complexes central for circadian clock and blue-light signaling, namely the WHITE-COLLAR complex (WCC) and FREQUENCY-interacting RNA helicase (FRH), a component that complexes with FRQ to mediate negative feedback control in Neurospora. WD interacts with FRH in the absence of WCC and FRQ but does not seem to control the exosome-mediated negative feedback loop. Instead, WD acts to modulate the transcriptional activity of the WCC.
机译:感光细胞和PAS / LOV蛋白VIVID(WD)调节蓝光信号并影响Neurospora crassa中生物钟的光和温度响应。 VVD对生物钟的主要作用之一是通过调节由中央生物钟基因频率(frq)编码的转录本的水平来影响生物钟相位。如何实现此调节尚不清楚。在这里,我们显示WD与昼夜节律和蓝光信号的中心复合物相互作用,即WHITE-COLLAR复合物(WCC)和FREQUENCY相互作用RNA解旋酶(FRH),该成分与FRQ复合以介导Neurospora中的负反馈控制。在没有WCC和FRQ的情况下,WD与FRH相互作用,但似乎无法控制外泌体介导的负反馈回路。相反,WD负责调节WCC的转录活性。

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