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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Escherichia coli condensin MukB stimulates topoisomerase IV activity by a direct physical interaction
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Escherichia coli condensin MukB stimulates topoisomerase IV activity by a direct physical interaction

机译:大肠杆菌凝缩蛋白MukB通过直接物理相互作用刺激拓扑异构酶IV活性

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摘要

In contrast to the current state of knowledge in the field of eukar yotic chromosome segregation, relatively little is known about the mechanisms coordinating the appropriate segregation of bacterial chromosomes. In Escherichia coli, the MukB/E/F complex and topoisomerase IV (Topo IV) are both crucial players in this process. Topo IV removes DNA entanglements following the replication of the chromosome, whereas MukB, a member of the structural maintenance of chromosomes protein family, serves as a bacterial condensin. We demonstrate here a direct physical interaction between the dimerization domain of MukB and the C-terminal domain of the ParC subunit of Topo IV. In addition, we find that MukB alters the activity of Topo IV in vitro. Finally, we isolate a MukB mutant, D692A, that is deficient in its interaction with ParC and show that this mutant fails to rescue the temperature-sensitive growth phenotype of a mukB~- strain. These results show that MukB and Topo IV are linked physically and functionally and indicate that the activities of these proteins are not limited to chro mosome segregation but likely also play a key role in the control of higher-order bacterial chromosome structure.
机译:与eukar y染色体染色体分离领域的当前知识水平相反,关于协调细菌染色体适当分离的机制知之甚少。在大肠杆菌中,MukB / E / F复合物和拓扑异构酶IV(Topo IV)在此过程中均至关重要。 Topo IV去除了染色体复制后的DNA缠结,而MukB(染色体蛋白质家族的结构维持成员)充当细菌凝缩蛋白。我们在这里证明MukB的二聚化域和Topo IV的ParC亚基的C末端域之间存在直接的物理相互作用。此外,我们发现MukB在体外改变了Topo IV的活性。最后,我们分离出了一个与ParC相互作用不足的MukB突变体D692A,并表明该突变体无法挽救mukB〜-菌株的温度敏感型生长表型。这些结果表明,MukB和Topo IV在物理上和功能上都相互连接,表明这些蛋白的活性不仅限于染色体分离,而且可能在控制高级细菌染色体结构中起关键作用。

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  • 作者单位

    Department of Chemistry, Indiana University, 212 South Hawthorne Street, Bloomington, IN 47405 Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY 10065;

    rnDepartment of Chemistry, Indiana University, 212 South Hawthorne Street, Bloomington, IN 47405;

    rnDepartment of Chemistry, Indiana University, 212 South Hawthorne Street, Bloomington, IN 47405;

    rnDepartment of Molecular and Cell Biology, California Institute for Quantitative Biology,University of California, Berkeley, CA 94720;

    rnDepartment of Molecular and Cell Biology, California Institute for Quantitative Biology,University of California, Berkeley, CA 94720;

    rnDepartment of Molecular and Cell Biology, California Institute for Quantitative Biology,University of California, Berkeley, CA 94720;

    rnLaboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY 10065;

    rnDepartment of Chemistry, Indiana University, 212 South Hawthorne Street, Bloomington, IN 47405-7102;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    bacterial chromosome segregation; mass spectrometry;

    机译:细菌染色体分离;质谱;

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