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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging
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Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging

机译:双歧双歧杆菌PRL2010的基因组分析揭示了宿主来源的聚糖觅食的代谢途径

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摘要

The human intestine is densely populated by a microbial consortium whose metabolic activities are influenced by, among others, bi-fidobacteria. However, the genetic basis of adaptation of bifido-bacteria to the human gut is poorly understood. Analysis of the 2,214,650-bp genome of Bifidobacterium bifidum PRL2010, a strain isolated from infant stool, revealed a nutrient-acquisition strategy that targets host-derived glycans, such as those present in mucin. Proteome and transcriptome profiling revealed a set of chromosomal loci responsible for mucin metabolism that appear to be under common transcriptional control and with predicted functions that allow degradation of various O-linked glycans in mucin. Conservation of the latter gene clusters in various B. bifidum strains supports the notion that host-derived glycan catabolism is an important colonization factor for B. bifidum with concomitant impact on intestinal microbiota ecology.
机译:人的肠由微生物聚居地密集分布,其代谢活性尤其受双歧杆菌的影响。但是,人们对双歧杆菌适应人类肠道的遗传基础了解甚少。从婴儿粪便分离出的双歧双歧杆菌PRL2010的2,214,650-bp基因组分析表明,营养获取策略以宿主来源的聚糖(如粘蛋白中存在的聚糖)为目标。蛋白质组和转录组分析显示了一组负责粘蛋白代谢的染色体基因座,这些基因座似乎处于共同的转录控制之下,并且具有预测的功能,可以降解粘蛋白中的各种O-连接聚糖。在各种双歧双歧杆菌菌株中后一种基因簇的保守性支持以下观点,即宿主衍生的聚糖分解代谢是双歧双歧杆菌的重要定居因子,同时对肠道菌群生态产生影响。

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  • 作者单位

    Laboratory of Probiogenomics, Department of Genetics, Biology of Microorganisms, Anthropology and Evolution, University of Parma, 43100 Parma, Italy;

    Laboratory of Probiogenomics, Department of Genetics, Biology of Microorganisms, Anthropology and Evolution, University of Parma, 43100 Parma, Italy,Alimentary Pharmabiotic Centre,Department of Microbiology, Bioscience Institute, National University of Ireland, Cork, Ireland;

    Laboratory of Probiogenomics, Department of Genetics, Biology of Microorganisms, Anthropology and Evolution, University of Parma, 43100 Parma, Italy;

    The Rowett Institute of Nutrition and Health, College of Life Sciences and Medicine, University of Aberdeen, Aberdeen, AB21 9SB United Kingdom;

    Department of Viticulture and Enology, The Robert Mondavi Institute for Wine and Food Sciences, University of California, Davis, CA 95616;

    Alimentary Pharmabiotic Centre;

    Departamento de Microbiologia y Bioquimica de Productos Lacteos, Instituto de Productos Lacteos de Asturias Consejo Superior de Investigaciones Cientificas, Villaviciosa, 3300 Asturias, Spain;

    Gepartment of Molecular Biology, University of Siena, 53100 Siena, Italy;

    Dipartimento Sientifico Tecnologico, Universita degli Studi di Verona, 37134 Verona, Italy;

    Laboratory of Probiogenomics, Department of Genetics, Biology of Microorganisms, Anthropology and Evolution, University of Parma, 43100 Parma, Italy;

    Dipartimento Sientifico Tecnologico, Universita degli Studi di Verona, 37134 Verona, Italy;

    Glycogenomics, Databases and Bioinformatics, Architecture et Fonction des Macromolecules Biologiques, Universites Aix-Marseille, 13288 Marseille, France;

    Glycogenomics, Databases and Bioinformatics, Architecture et Fonction des Macromolecules Biologiques, Universites Aix-Marseille, 13288 Marseille, France;

    Gepartment of Molecular Biology, University of Siena, 53100 Siena, Italy;

    Alimentary Pharmabiotic Centre,Department of Microbiology, Bioscience Institute, National University of Ireland, Cork, Ireland;

    Department of Viticulture and Enology, The Robert Mondavi Institute for Wine and Food Sciences, University of California, Davis, CA 95616;

    Departamento de Microbiologia y Bioquimica de Productos Lacteos, Instituto de Productos Lacteos de Asturias Consejo Superior de Investigaciones Cientificas, Villaviciosa, 3300 Asturias, Spain;

    The Rowett Institute of Nutrition and Health, College of Life Sciences and Medicine, University of Aberdeen, Aberdeen, AB21 9SB United Kingdom;

    Alimentary Pharmabiotic Centre,Department of Microbiology, Bioscience Institute, National University of Ireland, Cork, Ireland;

    Laboratory of Probiogenomics, Department of Genetics, Biology of Microorganisms, Anthropology and Evolution, University of Parma, 43100 Parma, Italy;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    coevolution; genomics; host-glycans metabolism; human gut intestinal bacteria; mucin;

    机译:协同进化基因组学宿主糖代谢人肠肠道细菌;粘蛋白;

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