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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Association mapping of local climate-sensitive quantitative trait loci in Arabidopsis thaliana
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Association mapping of local climate-sensitive quantitative trait loci in Arabidopsis thaliana

机译:拟南芥当地气候敏感数量性状基因座的关联图谱

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摘要

Flowering time (FT) is the developmental transition coupling an internal genetic program with external local and seasonal climate cues. The genetic loci sensitive to predictable environmental signals underlie local adaptation. We dissected natural variation in FT across a new global diversity set of 473 unique accessions, with > 12,000 plants across two seasonal plantings in each of two simulated local climates, Spain and Sweden. Genome-wide association mapping was carried out with 213,497 SNPs. A total of 12 FT candidate quantitative trait loci (QTL) were fine-mapped in two independent studies, including 4 located within ±10 kb of previously cloned FT alleles and 8 novel loci. All QTL show sensitivity to planting season and/or simulated location in a multi-QTL mixed model. Alleles at four QTL were significantly correlated with latitude of origin, implying past selection for faster flowering in southern locations. Finally,maximum seed yield was observed at an optimal FT unique to each season and location, with four FT QTL directly controlling yield. Our results suggest that these major, environmentally sensitive FT QTL play an important role in spatial and temporal adaptation.
机译:开花时间(FT)是将内部遗传程序与外部本地和季节性气候线索相结合的发展过渡。对可预测环境信号敏感的遗传基因座是局部适应的基础。我们在473个独特的新全球多样性中剖析了FT的自然变化,在西班牙和瑞典这两个模拟的本地气候中,每个季节都有超过12,000株植物。全基因组关联映射使用213,497个SNP进行。在两项独立的研究中对总共12个FT候选定量性状基因座(QTL)进行了精细映射,包括4个位于先前克隆的FT等位基因±10 kb内的基因和8个新基因座。在多个QTL混合模型中,所有QTL均显示出对种植季节和/或模拟位置的敏感性。四个QTL的等位基因与来源纬度显着相关,这意味着过去选择南部地区开花较快。最后,在每个季节和每个地点唯一的最佳FT下观察到最大种子产量,其中四个FT QTL直接控制产量。我们的结果表明,这些主要的,对环境敏感的FT QTL在时空适应中起着重要作用。

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