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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Force Amplification Response Of Actin Filaments Under Confined Compression
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Force Amplification Response Of Actin Filaments Under Confined Compression

机译:约束压缩下肌动蛋白丝的力放大响应

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摘要

Actin protein is a major component of the cell cytoskeleton, and its ability to respond to external forces and generate propulsive forces through the polymerization of filaments is central to many cellular processes. The mechanisms governing actin's abilities are still not fully understood because of the difficulty in observing these processes at a molecular level. Here, we describe a technique for studying actin-surface interactions by using a surface forces apparatus that is able to directly visualize and quantify the collective forces generated when layers of noninterconnected, end-tethered actin filaments are confined between 2 (mica) surfaces. We also identify a force-response mechanism in which filaments not only stiffen under compression, which increases the bending modulus, but more importantly generates opposing forces that are larger than the compressive force. This elastic stiffening mechanism appears to require the presence of confining surfaces, enabling actin filaments to both sense and respond to compressive forces without additional mediating proteins, providing insight into the potential role compressive forces play in many actin and other motor protein-based phenomena.
机译:肌动蛋白是细胞骨架的主要组成部分,它通过细丝的聚合反应外力并产生推进力的能力是许多细胞过程的核心。由于难以在分子水平上观察这些过程,因此仍无法完全理解控制肌动蛋白能力的机制。在这里,我们描述了一种通过使用表面力设备来研究肌动蛋白-表面相互作用的技术,该设备能够直接可视化并量化当非互连的,末端束缚的肌动蛋白丝层限制在2个(云母)表面之间时产生的集体力。我们还确定了一种力响应机制,其中长丝不仅在压缩下变硬,从而增加了弯曲模量,更重要的是产生了比压缩力大的相反力。这种弹性的增强机制似乎需要存在约束表面,从而使肌动蛋白丝能够感知并响应压缩力而无需其他介导蛋白,从而洞察了压缩力在许多肌动蛋白和其他基于运动蛋白的现象中的潜在作用。

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