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Deadly Competition Between Sibling Bacterial Colonies

机译:同胞细菌菌落之间的致命竞争

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摘要

Bacteria can secrete a wide array of antibacterial compounds when competing with other bacteria for the same resources. Some of these compounds, such as bacteriocins, can affect bacteria of similar or closely related strains. In some cases, these secretions have been found to kill sibling cells that belong to the same colony. Here, we present experimental observations of competition between 2 sibling colonies of Paenibacillus dendritiformis grown on a low-nutrient agar gel. We find that neighboring colonies (growing from droplet inoculation) mutually inhibit growth through secretions that become lethal if the level exceeds a well-defined threshold. In contrast, within a single colony developing from a droplet inoculation, no growth inhibition is observed. However, growth inhibition and cell death are observed if material extracted from the agar between 2 growing colonies is introduced outside a growing single colony. To interpret the observations, we devised a simple mathematical model for the secretion of an antibacterial compound. Simulations of this model illustrate how secretions from neighboring colonies can be deadly, whereas secretions from a single colony growing from a droplet are not.
机译:与其他细菌争夺相同资源时,细菌可以分泌多种抗菌化合物。其中一些化合物,例如细菌素,可影响相似或密切相关菌株的细菌。在某些情况下,已经发现这些分泌物杀死了属于同一菌落的兄弟细胞。在这里,我们提出了在低营养琼脂凝胶上生长的树状芽孢杆菌的两个同胞菌落之间竞争的实验观察。我们发现相邻的菌落(从液滴接种中生长)通过分泌物相互抑制生长,如果水平超过明确定义的阈值,这些分泌物将致死。相反,在由液滴接种形成的单个菌落内,未观察到生长抑制。但是,如果将从2个生长菌落之间的琼脂提取的物质引入到生长的单个菌落之外,则会观察到生长抑制和细胞死亡。为了解释观察结果,我们设计了一个简单的数学模型来分泌抗菌化合物。该模型的仿真说明了相邻菌落的分泌如何致命,而液滴生长的单个菌落的分泌却没有。

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