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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The Yeast Sup35nm Domain Propagates As A Prionin Mammalian Cells
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The Yeast Sup35nm Domain Propagates As A Prionin Mammalian Cells

机译:酵母Sup35nm域作为Prionin哺乳动物细胞传播。

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摘要

Prions are infectious, self-propagating amyloid-like protein aggregates of mammals and fungi. We have studied aggregation propensities of a yeast prion domain in cell culture to gain insights into general mechanisms of prion replication in mammalian cells. Here, we report the artificial transmission of a yeast prion across a phylogenetic kingdom. HA epitope-tagged yeast Sup35p prion domain NM was stably expressed in murine neuroblastoma cells. Although cytosoli-cally expressed NM-HA remained soluble, addition of fibrils of bac-terially produced Sup35NM to the medium efficiently induced appearance of phenotypically and biochemically distinct NM-HA aggregates that were inherited by daughter cells. Importantly, NM-HA aggregates also were infectious to recipient mammalian cells expressing soluble NM-HA and, to a lesser extent, to yeast. The fact that the yeast Sup35NM domain can propagate as a prion in neuroblastoma cells strongly argues that cellular mechanisms support prion-like inheritance in the mammalian cytosol.
机译:ions病毒是哺乳动物和真菌的传染性,自我繁殖的淀粉样蛋白聚集体。我们已经研究了细胞培养中酵母病毒域的聚集倾向,以了解into病毒在哺乳动物细胞中复制的一般机制。在这里,我们报告了酵母病毒在整个系统发育王国中的人工传播。 HA抗原表位标记的酵母Sup35p ion病毒域NM在鼠神经母细胞瘤细胞中稳定表达。尽管胞浆表达的NM-HA仍然可溶,但向培养基中添加细菌产生的Sup35NM的原纤维可有效诱导表型和生化性质不同的NM-HA聚集体的出现,这些聚集体是由子代细胞继承的。重要的是,NM-HA聚集体还可以感染表达可溶性NM-HA的受体哺乳动物细胞,并且在较小程度上对酵母具有感染性。酵母Sup35NM结构域可以作为a病毒在神经母细胞瘤细胞中繁殖的事实强烈表明,细胞机制支持哺乳动物细胞溶胶中病毒样遗传。

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  • 作者单位

    lnstitute of Virology, Technische Universitat Munchen, Trogerstrasse 30, 81675 Munich, Germany;

    Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892;

    Lehrstuhl fur Biomaterialien, Gebaude FAN/D, Universitat Bayreuth, Universitatsstrasse 30, 95447 Bayreuth, Germany;

    Institute of Molecular Immunology, Helmholtz Zentrum Munchen, Marchioninistrasse 25, 81377 Munich, Germany;

    Institute of Pharmacology and Toxicology, Universitat Bonn, Reuterstrasse 2b, 53113 Bonn, Germany;

    Institute of Pharmacology and Toxicology, Universitat Bonn, Reuterstrasse 2b, 53113 Bonn, Germany;

    Lehrstuhl fur Biomaterialien, Gebaude FAN/D, Universitat Bayreuth, Universitatsstrasse 30, 95447 Bayreuth, Germany;

    Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892;

    lnstitute of Virology, Technische Universitat Munchen, Trogerstrasse 30, 81675 Munich, Germany;

    lnstitute of Virology, Technische Universitat Munchen, Trogerstrasse 30, 81675 Munich, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    PRP; sup35;

    机译:PRP;sup35;

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