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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Multimodal Neuroimaging Provides A Highly Consistent Picture Of Energy Metabolism, Validating ~(31)p Mrs For Measuring Brain Atp Synthesis
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Multimodal Neuroimaging Provides A Highly Consistent Picture Of Energy Metabolism, Validating ~(31)p Mrs For Measuring Brain Atp Synthesis

机译：多峰神经影像学提供了能量代谢的高度一致的图像，验证了〜（31）p Mrs用于测量脑atp合成

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摘要

Neuroimaging methods have considerably developed over the last decades and offer various noninvasive approaches for measuring cerebral metabolic fluxes connected to energy metabolism, including PET and magnetic resonance spectroscopy (MRS). Among these methods, ~(31)P MRS has the particularity and advantage to directly measure cerebral ATP synthesis without injection of labeled precursor. However, this approach is methodologically challenging, and further validation studies are required to establish ~(31)P MRS as a robust method to measure brain energy synthesis. In the present study, we performed a multimodal imaging study based on the combination of 3 neuroimaging techniques, which allowed us to obtain an integrated picture of brain energy metabolism and, at the same time, to validate the saturation transfer ~(31)P MRS method as a quantitative measurement of brain ATP synthesis. A total of 29 imaging sessions were conducted to measure glucose consumption (CMRglc), TCA cycle flux (V_(TCA)), and the rate of ATP synthesis (Vatp) in primate monkeys by using ~(18)f-FDG PET scan, indirect ~(13)C MRS, and saturation transfer ~(31)P MRS, respectively. These 3 complementary measurements were performed within the exact same area of the brain under identical physiological conditions, leading to: CMRglc = 0.27 ± 0.07 μmol·g~(-1)·min~(-1), V_(TCA) = 0.63 ± 0.12 μmol·g~(-1)·min~(-1), and V_(ATP) = 7.8 ± 2.3 μmol·g~(-1)·min~(-1). The consistency of these 3 fluxes with literature and, more interestingly, one with each other, demonstrates the robustness of saturation transfer ~(31)P MRS for directly evaluating ATP synthesis in the living brain.
机译：在过去的几十年中，神经成像方法得到了长足的发展，并提供了各种非侵入性方法来测量与能量代谢相关的脑代谢通量，包括PET和磁共振波谱（MRS）。在这些方法中，〜（31）P MRS具有无需注射标记前体即可直接测量脑ATP合成的特殊性和优势。但是，这种方法在方法上具有挑战性，需要进一步的验证研究以建立〜（31）P MRS作为测量脑能量合成的可靠方法。在本研究中，我们基于3种神经成像技术的结合进行了多模态成像研究，这使我们能够获得脑能量代谢的完整图像，同时还能验证饱和转移〜（31）P MRS方法作为定量测量脑ATP合成的方法。通过〜（18）f-FDG PET扫描，共进行了29次成像会议以测量灵长类猴子的葡萄糖消耗（CMRglc），TCA循环通量（V_（TCA））和ATP合成速率（Vatp），间接〜（13）C MRS和饱和转移〜（31）P MRS。在相同的生理条件下，在完全相同的大脑区域内进行这3次互补测量，得出：CMRglc = 0.27±0.07μmol·g〜（-1）·min〜（-1），V_（TCA）= 0.63± 0.12μmol·g·（-1）·min·（-1），V_（ATP）＝ 7.8±2.3μmol·g·（-1）·min·（-1）。这三种通量与文献的一致性，以及更有趣的是彼此之间的一致性，证明了饱和转移〜（31）P MRS可直接评估活体大脑中ATP合成的鲁棒性。

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