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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Gene expression map of the Arabidopsis shoot apical meristem stem cell niche
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Gene expression map of the Arabidopsis shoot apical meristem stem cell niche

机译：拟南芥茎尖分生组织干细胞生态位基因表达图谱

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摘要

Despite the central importance of stem cells in plant growth and development, the molecular signatures associated with them have not been revealed. Shoot apical meristems (SAMs) harbor a small set of stem cells located at the tip of each plant and they are surrounded by several million differentiating cells. This imposes a major limitation in isolating pure populations of stem cells for genomic analyses. We have developed a system to isolate pure populations of distinct cell types of the SAMs, including stem cells. We have used this system to profile gene expression from 4 different cell samples of SAMs. The cell sample-specific gene expression profiling has resulted in a high-resolution gene expression map to reveal gene expression networks specific to individual spatial domains of SAMs. We demonstrate that the cell sample-specific expression profiling is sensitive in identifying rare transcripts expressed in a few specific subsets of cells of SAMs. Our extensive RNA in situ analysis reveals that the expression map can be used as a predictive tool in analyzing the spatial expression patterns of genes and it has led to the identification of unique gene expression patterns within the SAMs. Furthermore, our work reveals an enrichment of DNA repair and chromatin modification pathways in stem cells suggesting that maintenance of genome stability and flexible chromatin may be crucial for stem cell function. The gene expression map should guide future reverse genetics experiments, high-resolution analyses of cell-cell communication networks and epigenetic modifications.
机译：尽管干细胞在植物生长和发育中至关重要，但尚未揭示与它们相关的分子标记。茎尖分生组织（SAM）包含位于每株植物顶端的一小组干细胞，周围有数百万个分化细胞。这对分离干细胞的纯群体进行基因组分析施加了主要限制。我们已经开发出一种系统来分离SAM的不同细胞类型（包括干细胞）的纯种群。我们已经使用该系统来分析来自SAMs的4种不同细胞样品的基因表达。细胞样品特异的基因表达谱已形成高分辨率的基因表达图谱，以揭示特定于SAM的单个空间域的基因表达网络。我们证明，细胞样品特异性表达谱对鉴定在SAMs细胞的一些特定子集中表达的稀有转录物很敏感。我们广泛的RNA原位分析表明，表达图谱可用作分析基因的空间表达模式的预测工具，并已导致在SAM中识别独特的基因表达模式。此外，我们的工作揭示了干细胞中DNA修复和染色质修饰途径的丰富，这表明维持基因组稳定性和灵活的染色质可能对干细胞功能至关重要。基因表达图谱应指导未来的逆向遗传学实验，细胞间通信网络的高分辨率分析和表观遗传修饰。

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    Ram Kishor Yadav; Thomas Girke; Sumana Pasala; Mingtang Xie; G. Venugopala Reddy;

  • 作者单位

    Center for Plant Cell Biology and Department of Botany and Plant Sciences, 2150 Batchelor Hall, University of California, Riverside, CA 92521;

    Center for Plant Cell Biology and Department of Botany and Plant Sciences, 2150 Batchelor Hall, University of California, Riverside, CA 92521;

    Center for Plant Cell Biology and Department of Botany and Plant Sciences, 2150 Batchelor Hall, University of California, Riverside, CA 92521;

    Center for Plant Cell Biology and Department of Botany and Plant Sciences, 2150 Batchelor Hall, University of California, Riverside, CA 92521;

    Center for Plant Cell Biology and Department of Botany and Plant Sciences, 2150 Batchelor Hall, University of California, Riverside, CA 92521;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    central zone; CLAVATA3; fluorescence-activated cell sorting; WUSCHEL;

    机译：中央区CLAVATA3;荧光激活细胞分选;武士;

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