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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Mechanism of replication machinery assembly as revealed by the DNA ligase-PCNA-DNA complex architecture
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Mechanism of replication machinery assembly as revealed by the DNA ligase-PCNA-DNA complex architecture

机译：DNA连接酶-PCNA-DNA复杂体系结构揭示了复制机制组装的机制

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摘要

The 3D structure of the ternary complex, consisting of DNA ligase, the proliferating cell nuclear antigen (PCNA) clamp, and DNA, was investigated by single-particle analysis. This report presents the structural view, where the crescent-shaped DNA ligase with 3 distinct domains surrounds the central DNA duplex, encircled by the closed PCNA ring, thus forming a double-layer structure with dual contacts between the 2 proteins. The relative orientations of the DNA ligase domains, which remarkably differ from those of the known crystal structures, suggest that a large domain rearrangement occurs upon ternary complex formation. A second contact was found between the PCNA ring and the middle adenylation domain of the DNA ligase. Notably, the map revealed a substantial DNA tilt from the PCNA ring axis. This structure allows us to propose a switching mechanism for the replication factors operating on the PCNA ring.
机译：通过单颗粒分析研究了由DNA连接酶，增殖细胞核抗原（PCNA）钳和DNA组成的三元复合物的3D结构。该报告提供了结构视图，其中具有3个不同结构域的新月形DNA连接酶围绕着中央DNA双链体，被封闭的PCNA环包围，从而形成了一种双层结构，两个蛋白质之间具有双重接触。 DNA连接酶结构域的相对方向与已知的晶体结构明显不同，这表明在三元复合物形成后会发生较大的结构域重排。在PCNA环和DNA连接酶的中间腺苷酸化结构域之间发现了第二次接触。值得注意的是，该图谱显示DNA从PCNA环轴明显倾斜。这种结构使我们可以为PCNA环上运行的复制因子提出一种切换机制。

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    Kouta Mayanagi; Shinichi Kiyonari; Mihoko Saito; Tsuyoshi Shirai; Yoshizumi lshino; Kosuke Morikawa;

  • 作者单位

    Medical Institute of Bioregulation, Kyushu University, and Institute for Bioinformatics Research and Development, Japan Science and Technology Agency, 3-1-1 Maidashi, Higashi-ku, Fukuoka-shi, Fukuoka 812-8582, Japan Institute for Protein Research, Osaka University, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan;

    Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University,and Institute for Bioinformatics Research and Development, Japan Science and Technology Agency, 6-10-1 Hakozaki, Higashi-ku, Fukuoka-shi, Fukuoka 812-8581, Japan;

    Nagahama Institute of Bio-Science and Technology and Institute for Bioinformatics Research and Development, Japan Science and Technology Agency, 1266 Tamura-cho, Nagahama, Shiga 526-0829, Japan;

    Nagahama Institute of Bio-Science and Technology and Institute for Bioinformatics Research and Development, Japan Science and Technology Agency, 1266 Tamura-cho, Nagahama, Shiga 526-0829, Japan;

    Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University,and Institute for Bioinformatics Research and Development, Japan Science and Technology Agency, 6-10-1 Hakozaki, Higashi-ku, Fukuoka-shi, Fukuoka 812-8581, Japan;

    Institute for Protein Research, Osaka University, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Sanban-cho, Chiyoda-ku, Tokyo 102-0075, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    DNA replication; electron microscopy; single-particle analysis; DNA sliding clamp; protein-DNA complex;

    机译：DNA复制;电子显微镜;单颗粒分析;DNA滑动夹;蛋白质-DNA复合物;

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