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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >GeoChip-based analysis of metabolic diversity of microbial communities at the Juan de Fuca Ridge hydrothermal vent
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GeoChip-based analysis of metabolic diversity of microbial communities at the Juan de Fuca Ridge hydrothermal vent

机译:基于GeoChip的胡安德富卡里奇热液喷口微生物群落代谢多样性分析

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摘要

Deep-sea hydrothermal vents are one of the most unique and fascinating ecosystems on Earth. Although phylogenetic diversity of vent communities has been extensively examined, their physiological diversity is poorly understood. In this study, a GeoChip-based, high-throughput metagenomics technology revealed dramatic differences in microbial metabolic functions in a newly grown protochimney (inner section, Proto-I; outer section, Proto-O) and the outer section of a mature chimney (4143-1) at the Juan de Fuca Ridge. Very limited numbers of functional genes were detected in Proto-I (113 genes), whereas much higher numbers of genes were detected in Proto-0 (504 genes) and 4143-1 (5,414 genes). Microbial functional genes/populations in Proto-O and Proto-I were substantially different (around 1% common genes), suggesting a rapid change in the microbial community composition during the growth of the chimney. Previously retrieved cbbL and cbbM genes involved in the Calvin Benson Bassham (CBB) cycle from deep-sea hydrothermal vents were predominant in Proto-O and 4143-1, whereas photosynthetic green-like cbbL genes were the major components in Proto-I. In addition, genes involved in methanogenesis, aerobic and anaerobic methane oxidation (e.g., ANME1 and ANME2), nitrification, denitrification, sulfate reduction, degradation of complex carbon substrates, and metal resistance were also detected. Clone libraries supported the GeoChip results but were less effective than the microarray in delineating microbial populations of low biomass. Overall, these results suggest that the hydrothermal microbial communities are metaboli-cally and physiologically highly diverse, and the communities appear to be undergoing rapid dynamic succession and adaptation in response to the steep temperature and chemical gradients across the chimney.
机译:深海热液喷口是地球上最独特,最迷人的生态系统之一。尽管已广泛研究了通风孔群落的系统发育多样性,但对其生理多样性知之甚少。在这项研究中,基于GeoChip的高通量宏基因组学技术揭示了新生长的原烟囱(内部部分,Proto-I;外部部分,Proto-O)和成熟的烟囱(外部)的微生物代谢功能存在显着差异。 4143-1)在胡安·德·富卡岭。在Proto-I(113个基因)中检测到的功能基因数量非常有限,而在Proto-0(504个基因)和4143-1(5,414个基因)中检测到的基因数量更多。 Proto-O和Proto-I中的微生物功能基因/种群存在显着差异(大约1%的通用基因),这表明在烟囱生长过程中微生物群落组成迅速变化。先前从深海热液喷口回收的涉及Calvin Benson Bassham(CBB)循环的cbbL和cbbM基因在Proto-O和4143-1中占主导地位,而光合作用的绿色cbbL基因则是Proto-I的主要组成部分。另外,还检测到涉及甲烷生成,好氧和厌氧甲烷氧化(例如,ANME1和ANME2),硝化,反硝化,硫酸盐还原,复杂碳底物降解和金属抗性的基因。克隆文库支持GeoChip结果,但在描述低生物量微生物种群方面不如微阵列有效。总体而言,这些结果表明,热液微生物群落在代谢和生理上高度多样化,并且响应于烟囱上陡峭的温度和化学梯度,这些群落似乎正在经历快速的动态演替和适应。

著录项

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  • 作者单位

    Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, People's Republic of China School of Life Sciences and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, People's Republic of China;

    Department of Marine and Earth Sciences, Tongji University, Shanghai 200092, People's Republic of China;

    School of Life Sciences, Xiamen University, Xiamen 361005, People's Republic of China;

    Department of Marine and Earth Sciences, Tongji University, Shanghai 200092, People's Republic of China;

    Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, People's Republic of China;

    School of Life Sciences, Xiamen University, Xiamen 361005, People's Republic of China;

    Department of Marine Sciences, University of Georgia, Athens, GA 30602;

    Institute for Environmental Genomics, Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019;

    Institute for Environmental Genomics, Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019;

    Institute for Environmental Genomics, Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019;

    Institute for Environmental Genomics, Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019;

    Institute for Environmental Genomics, Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019;

    Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, People's Republic of China School of Life Sciences and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, People's Republic of China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    metagenomics; microarrays; chimney; deep sea; dynamic;

    机译:元基因组学微阵列烟囱;深海;动态;

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