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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Designed amphiphilic peptide forms stable nanoweb, slowly releases encapsulated hydrophobic drug, and accelerates animal hemostasis
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Designed amphiphilic peptide forms stable nanoweb, slowly releases encapsulated hydrophobic drug, and accelerates animal hemostasis

机译：设计的两亲性肽形成稳定的纳米纤维网，缓慢释放封装的疏水性药物，并加速动物止血

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摘要

How do you design a peptide building block to make 2-dimentional nanowebs and 3-dimensional fibrous mats? This question has not been addressed with peptide self-assembling nanomaterials. This article describes a designed 9-residue peptide, N-Pro-Ser-Phe-Cys-Phe-Lys-Phe-Glu-Pro-C, which creates a strong fishnet-like nano-structure depending on the peptide concentrations and mechanical disruptions. This peptide is intramolecularly amphiphilic because of a single pair of ionic residues, Lys and Glu, at one end and nonionic residues, Phe, Cys, and Phe, at the other end. Circular dichroism and Fourier transform infrared spectroscopy analysis demonstrated that this peptide adopts stable β-turn and β-sheet structures and self-assembles into hierarchically arranged supramolecular aggregates in a concentration-dependent fashion, demonstrated by atomic force microscopy and electron microscopy. At high concentrations, the peptide dominantly self-assembled into globular aggregates that were extensively connected with each other to form "beads-on-a-thread" type nanofibers. These long nanofibers were extensively branched and overlapped to form a self-healing peptide hydrogel consisting of >99% water. This peptide can encapsulate the hydrophobic model drug pyrene and slowly release pyrene from coated microcrystals to liposomes. It can effectively stop animal bleeding within 30 s. We proposed a plausible model to interpret the intramolecular amphiphilic self-assembly process and suggest its importance for the future development of new biomaterials for drug delivery and regenerative medicine.
机译：您如何设计制造二维二维纳米纤维网和三维三维纤维毡的肽结构单元？肽自组装纳米材料尚未解决该问题。本文介绍了一种设计的9残基肽N-Pro-Ser-Phe-Cys-Phe-Lys-Phe-Glu-Pro-C，它会根据肽浓度和机械破坏产生强力的鱼网状纳米结构。该肽是分子内两亲性的，因为一端有一对离子残基Lys和Glu，另一端有非离子残基Phe，Cys和Phe。圆二色性和傅立叶变换红外光谱分析表明，该肽采用稳定的β-turn和β-sheet结构，并以浓度依赖的方式自组装成分层排列的超分子聚集体，这通过原子力显微镜和电子显微镜证实。在高浓度下，该肽主要自组装成球状聚集体，这些聚集体彼此广泛连接以形成“线上珠子”型纳米纤维。这些长的纳米纤维被广泛分支并重叠以形成由> 99％的水组成的自愈肽水凝胶。该肽可以包封疏水模型药物drug，并从包被的微晶体中缓慢释放pyr到脂质体。它可以在30 s内有效阻止动物出血。我们提出了一个合理的模型来解释分子内两亲性自组装过程，并提出了其对于药物输送和再生医学新生物材料的未来发展的重要性。

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    Liping Ruan; Hangyu Zhang; Hanlin Luo; Jingping Liu; Fushan Tang; Ying-Kang Shi; Xiaojun Zhao;

  • 作者单位

    West China Hospital Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital and State Key Lab of Biotherapy of Human Diseases, Sichuan University, Science Park 1, Ke Yuan 4th Street, Gao Peng Road, Hi-tech Industrial Development Zone, Chengdu, 610041 Sichuan, China;

    West China Hospital Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital and State Key Lab of Biotherapy of Human Diseases, Sichuan University, Science Park 1, Ke Yuan 4th Street, Gao Peng Road, Hi-tech Industrial Development Zone, Chengdu, 610041 Sichuan, China;

    West China Hospital Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital and State Key Lab of Biotherapy of Human Diseases, Sichuan University, Science Park 1, Ke Yuan 4th Street, Gao Peng Road, Hi-tech Industrial Development Zone, Chengdu, 610041 Sichuan, China;

    West China Hospital Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital and State Key Lab of Biotherapy of Human Diseases, Sichuan University, Science Park 1, Ke Yuan 4th Street, Gao Peng Road, Hi-tech Industrial Development Zone, Chengdu, 610041 Sichuan, China;

    West China Hospital Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital and State Key Lab of Biotherapy of Human Diseases, Sichuan University, Science Park 1, Ke Yuan 4th Street, Gao Peng Road, Hi-tech Industrial Development Zone, Chengdu, 610041 Sichuan, China;

    West China Medical School, West China Hospital, Sichuan University, Guo Xue Xiang 37, Chengdu, 610041 Sichuan, China;

    West China Hospital Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital and State Key Lab of Biotherapy of Human Diseases, Sichuan University, Science Park 1, Ke Yuan 4th Street, Gao Peng Road, Hi-tech Industrial Development Zone, Chengdu, 610041 Sichuan, China West China Medical School, West China Hospital, Sichuan University, Guo Xue Xiang 37, Chengdu, 610041 Sichuan, China Center for Biomedical Engineering, Massachusetts Institute of Technology, 500 Technology Square, Cambridge, MA 02139-4307;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    β-turn/β-sheet; hemostasis; nanofibers; hydrogel; self-assembly;

    机译：β-转角/β-折叠;止血纳米纤维水凝胶自组装;

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