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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Sumoylation of ABI5 by the Arabidopsis SUMO E3 ligase SIZ1 negatively regulates abscisic acid signaling
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Sumoylation of ABI5 by the Arabidopsis SUMO E3 ligase SIZ1 negatively regulates abscisic acid signaling

机译：拟南芥SUMO E3连接酶SIZ1对ABI5的糖基化负调控脱落酸信号。

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摘要

SUMO (small ubiquitin-related modifier) conjugation (i.e., sumoylation) to protein substrates is a reversible posttranslational modification that regulates signaling by modulating transcription factor activity. This paper presents evidence that the SUMO E3 ligase SIZ1 negatively regulates abscisic acid (ABA) signaling, which is dependent on the bZIP transcription factor ABI5. Loss-of-function T-DNA insertion siz1-2 and siz1-3 mutations caused ABA hyper-sensitivity for seed germination arrest and seedling primary root growth inhibition. Furthermore, expression of genes that are ABA-responsive through ABI5-dependent signaling (e.g., RD29A, Rd29B, AtEm6, RAB18. ADH1) was hyperinduced by the hormone in siz1 seedlings. abi5-4 suppressed ABA hypersensitivity caused by siz1 (siz1-2 abi5-4), demonstrating an epistatic genetic interaction between SIZ1 and ABI5. A K391R substitution in ABI5 [ABI5(K391R)] blocked SIZ1-mediated sumoylation of the transcription factor in vitro and in Arabidopsis protoplasts, indicating that ABI5 is sumoylated through SIZ1 and that K391 is the principal site for SUMO conjugation. In abi5-4 plants, ABI5(K391R) expression caused greater ABA hypersensitivity (gene expression, seed germination arrest and primary root growth inhibition) compared with ABI5 expression. Together, these results establish that SIZ1-dependent sumoylation of ABI5 attenuates ABA signaling. The double mutant siz1-2 afp-1 exhibited even greater ABA sensitivity than the single mutant siz1, suggesting that SIZ1 represses ABI5 signaling function independent of AFP1.
机译：SUMO（小的泛素相关修饰物）与蛋白质底物的缀合（即SUMO化）是可逆的翻译后修饰，其通过调节转录因子活性来调节信号传导。本文提供的证据表明，SUMO E3连接酶SIZ1负调控脱落酸（ABA）信号传导，这取决于bZIP转录因子ABI5。功能丧失的T-DNA插入siz1-2和siz1-3突变引起ABA对种子发芽停止和幼苗初级根生长抑制的超敏感性。此外，siz1幼苗中的激素会过度诱导通过ABI5依赖性信号转导ABA响应的基因的表达（例如RD29A，Rd29B，AtEm6，RAB18。ADH1）。 abi5-4抑制了由siz1（siz1-2 abi5-4）引起的ABA超敏反应，表明SIZ1和ABI5之间存在上位的遗传相互作用。在体外和拟南芥原生质体中，ABI5 [ABI5（K391R）]中的K391R取代阻止了SIZ1介导的转录因子的合成，这表明ABI5通过SIZ1被合成，并且K391是SUMO偶联的主要位点。与ABI5表达相比，在abi5-4植物中，ABI5（K391R）表达引起更大的ABA超敏反应（基因表达，种子萌发停止和初级根生长抑制）。在一起，这些结果确定ABI5 SIZ1依赖sumoylation减弱ABA信号传导。双突变体siz1-2 afp-1的ABA敏感性比单个突变体siz1更高，这表明SIZ1抑制了独立于AFP1的ABI5信号传导功能。

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    Kenji Miura; Jiyoung Lee; Jing Bo Jin; Chan Yul Yoo; Tomoko Miura; Paul M. Hasegawa;

  • 作者单位

    Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, IN 47907 Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan;

    Division of Applied Life Science (BK21 Program) and Environmental Biotechnology National Core Research Center, Graduate School of Gyeongsang National University, Jinju 660-701, Korea Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637;

    Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, IN 47907 Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;

    Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, IN 47907;

    Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, IN 47907;

    Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, IN 47907;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    abscisic acid; signaling; SUMO; sumoylation;

    机译：脱落酸发信号相扑;磺酰化;

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