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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A homolog of human ski-interacting protein in rice positively regulates cell viability and stress tolerance
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A homolog of human ski-interacting protein in rice positively regulates cell viability and stress tolerance

机译：水稻中与人滑雪相互作用蛋白的同系物正调节细胞活力和胁迫耐受性

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摘要

Abiotic stresses are major limiting factors for growth, development, and productivity of crop plants. Here, we report on OsSKlPa, a rice homolog of human Ski-interacting protein (SKIP) that can complement the lethal defect of the knockout mutant of SKIP homolog in yeast and positively modulate cell viability and stress tolerance of rice. Suppression of OsSKlPa in rice resulted in growth arrest and reduced cell viability. The expression OsSKlPa is induced by various abiotic stresses and phytohormone treatments. Trans-genic rice overexpressing OsSKlPa exhibited significantly improved growth performance in the medium containing stress agents (ab-scisic acid, salt, or mannitol) and drought resistance at both the seedling and reproductive stages. The OsSKIPa-overexpressing rice showed significantly increased reactive oxygen species-scavenging ability and transcript levels of many stress-related genes, including SNAC1 and rice homologs of CBF2, PP2C, and RD22, under drought stress conditions. More than 30 OsSKIPa-interacting proteins were identified, but most of these proteins have no matches with the reported SKIP-interacting proteins in animals and yeast. Together, these data suggest that OsSKlPa has evolved a specific function in positive modulation of stress resistance through tran-scriptional regulation of diverse stress-related genes in rice.
机译：非生物胁迫是作物生长，发育和生产力的主要限制因素。在这里，我们报道了OsSKlPa，这是一种人类滑雪相互作用蛋白（SKIP）的水稻同源物，可以与酵母中SKIP同源物的敲除突变体的致死缺陷互补，并正向调节水稻的细胞生存力和胁迫耐受性。水稻中OsSK1Pa的抑制导致生长停滞和细胞活力降低。 OsSK1Pa表达是由各种非生物胁迫和植物激素治疗诱导的。过表达OsSKlPa的转基因水稻在含有胁迫因子（ab-scisic acid，盐或甘露醇）的培养基中表现出显着改善的生长性能，在苗期和生殖期均表现出抗旱性。在干旱胁迫条件下，过表达OsSKIPa的水稻表现出显着提高的清除活性氧的能力和许多胁迫相关基因的转录水平，包括SNAC1和水稻同源的CBF2，PP2C和RD22。鉴定出了超过30种与OsSKIPa相互作用的蛋白，但这些蛋白中的大多数与动物和酵母中报道的与SKIP相互作用的蛋白均不匹配。总之，这些数据表明，OsSK1Pa通过转录调控水稻中与胁迫相关的各种基因而在逆境抗性的正调控中发挥了特定作用。

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  • 作者

    Xin Hou; Kabin Xie; Jialing Yao; Zhuyun Qi; Lizhong Xiong;

  • 作者单位

    National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China;

    National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China;

    National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China;

    National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China;

    National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    abiotic stress; Oryza sativa; SKIP; transcriptional regulation;

    机译：非生物胁迫水稻;跳跃;转录调控;

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