...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Plastid production of protein antibiotics against pneumonia via a new strategy for high-level expression of antimicrobial proteins
【24h】

Plastid production of protein antibiotics against pneumonia via a new strategy for high-level expression of antimicrobial proteins

机译：通过高水平表达抗菌素蛋白的新策略可塑性生产抗肺炎的蛋白抗生素

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Plastid transformation has become an attractive tool in biotechnology. Because of the prokaryotic nature of the plastid's gene expression machinery, expression elements (promoters and untranslated regions) that trigger high-level foreign protein accumulation in plastids usually also confer high expression in bacterial cloning hosts. This can cause problems, for example, when production of antimicrobial compounds is attempted. Their bactericidal activity can make the cloning of the corresponding genes in plastid transformation vectors impossible. Here, we report a general solution to this problem. We have designed a strategy (referred to as toxin shuttle) that allows the expression in plastids of proteins that are toxic to Escherichia coli. The strategy is based on blocking transcription in E. coli by bacterial transcription terminators upstream of the gene of interest, which subsequently are excised in planta by site-specific recombination. We demonstrate the applicability of the strategy by the high-level expression in plastids (to up to 30% of the plant's total soluble protein) of 2 phage-derived protein antibiotics that are toxic to E. coli. We also show that the plastid-produced antibiotics efficiently kill pathogenic strains of Streptococcus pneumoniae, the causative agent of pneumonia, thus providing a promising strategy for the production of next-generation antibiotics in plants.
机译：质体转化已成为生物技术中有吸引力的工具。由于质体基因表达机制的原核性质，触发质体中高水平外源蛋白积累的表达元件（启动子和非翻译区）通常还可以在细菌克隆宿主中实现高表达。例如，当尝试生产抗菌化合物时，这可能会引起问题。它们的杀菌活性使得不可能在质体转化载体中克隆相应的基因。在这里，我们报告此问题的一般解决方案。我们设计了一种策略（称为毒素穿梭），该策略可在质体中表达对大肠杆菌有毒的蛋白质。该策略基于目标基因上游的细菌转录终止子阻断大肠杆菌中的转录，随后通过位点特异性重组将其在植物中切除。我们通过对两种对大肠杆菌有毒的噬菌体衍生的蛋白抗生素在质体中高水平表达（最多占植物总可溶性蛋白的30％）来证明该策略的适用性。我们还表明，质体产生的抗生素有效地杀死了肺炎链球菌（肺炎的病原体）的致病菌株，从而为在植物中生产下一代抗生素提供了有希望的策略。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号