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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Isoenzyme replacement of glucose-6-phosphate dehydrogenase in the cytosol improves stress tolerance in plants
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Isoenzyme replacement of glucose-6-phosphate dehydrogenase in the cytosol improves stress tolerance in plants

机译：胞浆中葡萄糖-6-磷酸脱氢酶的同工酶替代提高植物的胁迫耐受性

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摘要

In source leaves of resistant tobacco, oxidative burst and subsequent formation of hypersensitive lesions after infection with Phytophthora nicotianae was prevented by inhibition of glucose-6-phosphate dehydrogenase (G6PDH) or NADPH oxidases. This observation indicated that plant defense could benefit from improved NADPH availability due to increased G6PDH activity in the cytosol. A plastidic isoform of the G6PDH-encoding gene, G6PD, displaying high NADPH tolerance was engineered for cytosolic expression (cP2), and introduced into a susceptible cultivar. After infection, transgenic (previously susceptible) lines overexpressing cP2 showed early oxidative bursts, callose deposition, and changes in metabolic parameters. These responses resulted in timely formation of hypersensitive lesions similar to resistant plants, although their extent varied considerably between different transgenic lines. Additional RNAi suppression of endogenous cytosolic G6PD isoforms resulted in highly uniform defense responses and also enhanced drought tolerance and flowering. Cytosolic G6PDH seems to be a crucial factor for the outcome of plant defense responses; thus, representing an important target for modulation of stress resistance. Because isoenzyme replacement of G6PDH in the cytosol was beneficial under various kinds of cues, we propose this strategy as a tool to enhance stress tolerance in general.
机译：在抗性烟草的源叶中，通过抑制葡萄糖-6-磷酸脱氢酶（G6PDH）或NADPH氧化酶，可以防止烟草疫霉感染后的氧化爆发和随后的过敏性病变的形成。该观察结果表明，由于细胞质中G6PDH活性的提高，植物防御可以受益于NADPH利用率的提高。 G6PDH编码基因G6PD的质体同工型显示出较高的NADPH耐受性，被改造用于胞质表达（cP2），并引入到易感品种中。感染后，过表达cP2的转基因（先前易感）品系显示出早期的氧化爆发，call质沉积和代谢参数的变化。这些反应导致及时形成与抗性植物相似的过敏性病变，尽管它们的程度在不同的转基因品系之间差异很大。内源性胞质G6PD亚型的其他RNAi抑制导致高度统一的防御反应，还增强了耐旱性和开花能力。胞质G6PDH似乎是植物防御反应结果的关键因素。因此，代表了抗应力调节的重要目标。因为在各种提示下，胞浆中G6PDH的同功酶替代都是有益的，所以我们建议将该策略作为一种总体上增强压力耐受性的工具。

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