...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >High frequency of cephalic neural crest cells shows coexistence of neurogenic, melanogenic, and osteogenic differentiation capacities
【24h】

High frequency of cephalic neural crest cells shows coexistence of neurogenic, melanogenic, and osteogenic differentiation capacities

机译：高频头神经neural细胞显示神经原，黑色素和成骨分化能力共存

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The neural crest (NC) is a vertebrate innovation that distinguishes vertebrates from other chordates and was critical for the development and evolution of a "New Head and Brain." In early vertebrates, the NC was the source of dermal armor of fossil jawless fish. In extant vertebrates, including mammals, the NC forms the peripheral nervous system, melanocytes, and the cartilage and bone of the face. Here, we show that in avian embryos, a large majority of cephalic NC cells (CNCCs) have the ability to differentiate into cell types as diverse as neurons, melanocytes, osteocytes, and chondrocytes. Moreover, we find that the mor-phogen Sonic hedgehog (Shh) acts on CNCCs to increase endo-chondral osteogenesis while having no effect on osteoblasts prone to membranous ossification. We have developed culture conditions that demonstrate that "neural-mesenchymal" differentiation abilities are present in more than 90% of CNCCs. A highly multipotent progenitor (able to yield neurons, glia, melanocytes, myofibroblasts, chondrocytes, and osteocytes) comprises 7-13% of the clonogenic cells in the absence and presence of Shh, respectively. This progenitor is a good candidate for a cephalic NC stem cell.
机译：神经rest（NC）是一种脊椎动物创新，将脊椎动物与其他脊索动物区分开来，并且对于“新头颅和大脑”的发展和进化至关重要。在早期的脊椎动物中，NC是无颚化石鱼的皮肤铠甲的来源。在现存的脊椎动物（包括哺乳动物）中，NC形成周围神经系统，黑素细胞以及脸部的软骨和骨骼。在这里，我们显示出在禽类胚胎中，大多数头型NC细胞（CNCC）具有分化为神经元，黑素细胞，骨细胞和软骨细胞等多种细胞类型的能力。此外，我们发现病原体声波刺猬（Shh）作用于CNCC，以增加软骨内成骨作用，而对易于发生膜化骨化的成骨细胞没有影响。我们已经开发出可以证明90％以上的CNCC存在“神经间质”分化能力的培养条件。在Shh不存在和存在下，高度多能的祖细胞（能够产生神经元，神经胶质细胞，黑素细胞，成肌纤维细胞，软骨细胞和骨细胞）分别占克隆细胞的7-13％。该祖细胞是头颅NC干细胞的良好候选者。

著录项

  • 来源
  • 作者

    Giordano W. Calloni; Nicole M. Le Douarin; Elisabeth Dupin;

  • 作者单位

    Centre National de la Recherche Scientifique Unite Propre de Recherche 2197 Laboratoire Developpement, Evolution et Plasticite du Systeme Nerveux,Institut de Neurobiologie Alfred Fessard, 91198 Gif-sur-Yvette, France;

    Centre National de la Recherche Scientifique Unite Propre de Recherche 2197 Laboratoire Developpement, Evolution et Plasticite du Systeme Nerveux,Institut de Neurobiologie Alfred Fessard, 91198 Gif-sur-Yvette, France;

    Centre National de la Recherche Scientifique Unite Propre de Recherche 2197 Laboratoire Developpement, Evolution et Plasticite du Systeme Nerveux,Institut de Neurobiologie Alfred Fessard, 91198 Gif-sur-Yvette, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    clonal culture; dermal bone; endochondral bone; multipotency; quail embryo;

    机译：克隆培养真皮骨软骨内骨多能鹌鹑胚胎;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号