IpaB-IpgC interaction defines binding motif for type Ⅲ secretion translocator

Michele Lunelli; Ravi Kumar Lokareddy; Arturo Zychlinsky; Michael Kolbe

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IpaB-IpgC interaction defines binding motif for type Ⅲ secretion translocator

机译：IpaB-IpgC相互作用定义了Ⅲ型分泌转运蛋白的结合基序

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The delivery of virulence factors into host cells through type Ⅲ secretion systems is essential for enterobacterial pathogenesis. Molecular chaperones bind specifically to virulence factors in the bacterial cytosol before secretion. Invasion plasmid gene C (IpgC) is a chaperone that binds 2 essential virulence factors of Shigella: invasion plasmid antigens (Ipa) B and C. Here, we report the crystal structure of IpgC alone and in complex with the chaperone binding domain (CBD) of IpaB. The chaperone captures the CBD in an extended conformation that is stabilized by conserved residues lining the cleft. Analysis of the cocrystal structure reveals a sequence motif that is functional in the IpaB translocator class from different bacteria as determined by isothermal titration calorime-try. Our results show how translocators are chaperoned and may allow the design of inhibitors of enterobacterial diseases.

机译：通过Ⅲ型分泌系统将毒力因子传递到宿主细胞中对于肠道细菌的发病机理至关重要。分子伴侣在分泌前特异性结合细菌细胞质中的毒力因子。侵袭质粒基因C（IpgC）是一种结合志贺氏菌2个基本毒力因子的伴侣蛋白：侵袭质粒抗原（Ipa）B和C。在这里，我们报道了IpgC的晶体结构，并且与伴侣结合结构域（CBD）复杂IpaB。分子伴侣以扩展的构象捕获CBD，该构象通过裂口内的保守残基稳定。对共晶体结构的分析揭示了一个序列基序，该序列基序在等温滴定量热法测定的不同细菌的IpaB易位子类别中起作用。我们的研究结果表明易位分子是如何被伴侣化的，并且可能允许设计肠细菌性疾病的抑制剂。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2009年第24期|9661-9666|共6页
作者
Michele Lunelli; Ravi Kumar Lokareddy; Arturo Zychlinsky; Michael Kolbe;
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作者单位

Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Chariteplatz 1, 10117 Berlin, Germany;

Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Chariteplatz 1, 10117 Berlin, Germany;

Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Chariteplatz 1, 10117 Berlin, Germany;

Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Chariteplatz 1, 10117 Berlin, Germany;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
asymmetric homodimer; chaperone; microbiology; pathogens; tetratricopeptide repeat;

机译：不对称同二聚体伴侣微生物学;病原体四肽重复;

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1. Combination of Two Separate Binding Domains Defines Stoichiometry between Type III Secretion System Chaperone IpgC and Translocator Protein IpaB [J] . Ravi Kumar Lokareddy, Michele Lunelli, Bj?rn Eilers, The Journal of biological chemistry . 2010,第51期

机译：两种单独的结合结构域的组合在III型分泌系统伴随IPGC和译备器蛋白IPAB之间定义了化学计量
2. BipC, a Predicted Burkholderia pseudomallei Type 3 Secretion System Translocator Protein with Actin Binding Activity [J] . Vander Broek Charles W., Zainal Abidin Nurhamimah, Stevens Joanne M. Frontiers in Cellular and Infection Microbiology . 2017,第2016期

机译：BipC，一种具有肌动蛋白结合活性的预测的伯克霍尔德氏假疟原虫3型分泌系统转运蛋白
3. Mapping of the chaperone AcrH binding regions of translocators AopB and AopD and characterization of oligomeric and metastable AcrH-AopB-AopD complexes in the type III secretion system of Aeromonas hydrophila. [J] . Tan YW, Yu HB, Sivaraman J Protein Science: A Publication of the Protein Society . 2009,第8期

机译：嗜水气单胞菌III型分泌系统中易位蛋白AopB和AopD的伴侣AcrH结合区的定位以及寡聚和亚稳的AcrH-AopB-AopD复合物的表征。
4. NMR solution structure of B-motif,a signature motif of type-B response regulators for his-to-asp phosphorelay signal transduction system,and its interactions with DNA [C] . Kazuo Hosoda, Etsuko Katoh, Tomohisa Hatta, the International Peptide Symposium . 2001

机译：B-MOTIF的NMR溶液结构，B型响应调节剂的签名基序，其用于他 - ASP磷光素信号转导系统及其与DNA的相互作用
5. The Pseudomonas syringae type III secretion system: The translocator proteins, their secretion, and the restriction of translocation by the plant immune system [D] . Crabill, Emerson 2012

机译：丁香假单胞菌III型分泌系统：易位蛋白，其分泌以及植物免疫系统对易位的限制
6. IpaB–IpgC interaction defines binding motif for type III secretion translocator [O] . Michele Lunelli, Ravi Kumar Lokareddy, Arturo Zychlinsky, 2009

机译：IpaB–IpgC相互作用定义了III型分泌转运蛋白的结合基序
7. IpaB–IpgC interaction defines binding motif for type III secretion translocator [O] . Lunelli, Michele, Lokareddy, Ravi Kumar, Zychlinsky, Arturo, 2009

机译：IpaB–IpgC相互作用定义了III型分泌转运蛋白的结合基序

IpaB-IpgC interaction defines binding motif for type Ⅲ secretion translocator

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