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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Scaffold expulsion and genome packaging trigger stabilization of herpes simplex virus capsids
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Scaffold expulsion and genome packaging trigger stabilization of herpes simplex virus capsids

机译：支架驱逐和基因组包装触发单纯疱疹病毒衣壳的稳定

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摘要

Herpes simplex virus type 1 (HSV1) capsids undergo extensive structural changes during maturation and DNA packaging. As a result, they become more stable and competent for nuclear egress. To further elucidate this stabilization process, we used biochemical and nanoindentation approaches to analyze the structural and mechanical properties of scaffold-containing (B), empty (A), and DNA-containing (C) nuclear capsids. Atomic force microscopy experiments revealed that A and C capsids were mechanically indistinguishable, indicating that the presence of DNA does not account for changes in mechanical properties during capsid maturation. Despite having the same rigidity, the scaffold-containing B capsids broke at significantly lower forces than A and C capsids. An extraction of pentons with guanidine hydrochloride (GuHCl) increased the flexibility of all capsids. Surprisingly, the breaking forces of the modified A and C capsids dropped to similar values as those of the GuHCI-treated B capsids, indicating that mechanical reinforcement occurs at the vertices. Nonetheless, it also showed that HSV1 capsids possess a remarkable structural integrity that was preserved after removal of pentons. We suggest that HSV1 capsids are stabilized after removal of the scaffold proteins, and that this stabilization is triggered by the packaging of DNA, but independent of the actual presence of DNA.
机译：1型单纯疱疹病毒（HSV1）衣壳在成熟和DNA包装过程中会发生广泛的结构变化。结果，它们变得更加稳定并有能力进行核出口。为了进一步阐明这种稳定过程，我们使用了生物化学和纳米压痕方法来分析包含支架的（B），空的（A）和包含DNA的（C）核衣壳的结构和机械性能。原子力显微镜实验显示，A和C衣壳在机械上是无法区分的，这表明DNA的存在不能解释衣壳成熟过程中机械性能的变化。尽管具有相同的刚度，但包含支架的B衣壳以比A和C衣壳低得多的力断裂。用盐酸胍（GuHCl）提取五聚体增加了所有衣壳的柔韧性。出人意料的是，修饰的A和C衣壳的断裂力下降到与GuHCI处理的B衣壳的断裂力相似的值，表明机械增强发生在顶点。尽管如此，它还表明，HSV1衣壳具有出色的结构完整性，该结构完整性在去除戊烯后得以保留。我们建议去除支架蛋白后，HSV1衣壳被稳定化，这种稳定化是由DNA包装触发的，但与DNA的实际存在无关。

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