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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Elimination of plasmatocytes by targeted apoptosis reveals their role in multiple aspects of the Drosophila immune response
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Elimination of plasmatocytes by targeted apoptosis reveals their role in multiple aspects of the Drosophila immune response

机译：通过靶向凋亡消除浆细胞表明其在果蝇免疫应答的多个方面

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摘要

Drosophila hemocytes have strong phagocytic capacities and produce antimicrobial peptides (AMPs). However, the precise role of blood cells during immune responses and developmental processes has only been studied using indirect means. To overcome this limitation, we generated plasmatocyte-depleted flies by specifically overexpressing the proapoptotic protein Hid into plasmatocytes. Unexpectedly, these plasmatocyte-depleted animals have a normal larval and pupal development and do not exhibit any obvious defect after birth. Remarkably, plasmatocyte-depleted adults show a strong susceptibility to infections by various microorganisms, although activation of systemic AMP gene transcription via the Toll and immune deficiency (IMD) pathways is wild-type. Our data show that this susceptibility, which correlates with overproliferation of bacteria, is likely due to the absence of phagocytosis. We also demonstrate that during larval stages, plasmatocytes play an essential role in mediating AMP production by the fat body after oral bacterial infection. Finally, we show that plasmatocytes are involved in immune surveillance during pupal development, because they prevent bacterial infection that causes pupal lethality.
机译：果蝇血细胞具有很强的吞噬能力，​​并能产生抗菌肽（AMP）。但是，血细胞在免疫反应和发育过程中的确切作用仅是通过间接手段研究的。为了克服这一局限性，我们通过特异性地将促凋亡蛋白Hid过度表达到浆细胞中来生成贫浆细胞蝇。出乎意料的是，这些贫血的贫血动物的幼虫和development发育正常，出生后没有任何明显的缺陷。值得注意的是，尽管通过Toll和免疫缺陷（IMD）途径激活的系统AMP基因转录是野生型，但贫血的成人成年人对各种微生物的感染表现出强烈的敏感性。我们的数据表明，这种易感性与细菌的过度增殖有关，很可能是由于缺乏吞噬作用。我们还证明，在幼虫阶段，浆细胞在口腔细菌感染后在由脂肪体介导AMP产生中起重要作用。最后，我们表明浆细胞参与p发育过程中的免疫监视，因为它们可以防止引起infection致死性的细菌感染。

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  • 作者

    Bernard Charroux; Julien Royet;

  • 作者单位

    Institut de Biologie du Developpement de Marseille Luminy, Unite Mixte de Recherche 6216, Centre National de la Recherche Scientifique, Universite de la Mediterrannee Aix-Marseille II, 13288 Marseille Cedex 9, France;

    Institut de Biologie du Developpement de Marseille Luminy, Unite Mixte de Recherche 6216, Centre National de la Recherche Scientifique, Universite de la Mediterrannee Aix-Marseille II, 13288 Marseille Cedex 9, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    AMP; blood cells; innate immunity; toll;

    机译：AMP;血细胞;先天免疫;收费;

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