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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Intracellular Ca~(2+) signaling and store-operated Ca~(2+) entry are required in Drosophila neurons for flight
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Intracellular Ca~(2+) signaling and store-operated Ca~(2+) entry are required in Drosophila neurons for flight

机译：果蝇神经元需要细胞内Ca〜（2+）信号传导和存储操作的Ca〜（2+）进入

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摘要

Neuronal Ca~(2+) signals can affect excitability and neural circuit formation. Ca~(2+) signals are modified by Ca~(2+) flux from intracellular stores as well as the extracellular milieu. However, the contribution of intracellular Ca~(2+) stores and their release to neuronal processes is poorly understood. Here, we show by neuron-specific siRNA depletion that activity of the recently identified store-operated channel encoded by dOrai and the endoplasmic reticulum Ca~(2+) store sensor encoded by dSTIM are necessary for normal flight and associated patterns of rhythmic firing of the flight motoneurons of Drosophila melanogaster. Also, dOrai overexpression in flightless mutants for the Drosophila inositol 1,4,5-trisphosphate receptor (InsP_3R) can partially compensate for their loss of flight. Ca~(2+) measurements show that Orai gain-of-function contributes to the quanta of Ca~(2+)-release through mutant InsP_3Rs and elevates store-operated Ca~(2+) entry in Drosophila neurons. Our data show that replenishment of intracellular store Ca~(2+) in neurons is required for Drosophila flight.
机译：神经元Ca〜（2+）信号可影响兴奋性和神经回路形成。 Ca〜（2+）信号被细胞内存储以及细胞外环境中的Ca〜（2+）通量修饰。然而，人们对细胞内Ca〜（2+）存储及其释放对神经元过程的贡献了解甚少。在这里，我们通过神经元特异的siRNA消耗表明，dOrai编码的最近鉴定的存储操作通道和dSTIM编码的内质网Ca〜（2+）存储传感器的活性对于正常飞行和相关的有节奏的发射有必要果蝇的飞行运动神经元。此外，果蝇肌醇1,4,5-三磷酸受体（InsP_3R）的不会飞行的突变体中的dOrai过表达可以部分弥补其飞行损失。 Ca〜（2+）的测量结果表明，Orai功能获得通过突变InsP_3Rs促进Ca〜（2+）释放的数量，并提高了果蝇神经元中存储操作的Ca〜（2+）的进入。我们的数据表明果蝇飞行需要补充神经元中的细胞内存储Ca〜（2+）。

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