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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Control of retinal isomerization in bacteriorhodopsin in the high-intensity regime
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Control of retinal isomerization in bacteriorhodopsin in the high-intensity regime

机译:高强度状态下细菌视紫红质的视网膜异构化控制

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摘要

A learning algorithm was used to manipulate optical pulse shapes and optimize retinal isomerization in bacteriorhodopsin, for excitation levels up to 1.8 x 10~(16) photons per square centimeter. Below 1/3 the maximum excitation level, the yield was not sensitive to pulse shape. Above this level the learning algorithm found that a Fourier-transform-limited (TL) pulse maximized the 13-cis population. For this optimal pulse the yield increases linearly with intensity well beyond the saturation of the first excited state. To understand these results we performed systematic searches varying the chirp and energy of the pump pulses while monitoring the isomerization yield. The results are interpreted including the influence of 1-photon anc multiphoton transitions. The population dynamics in each intermediate conformation and the final branching ratio between the all-trans and 13-cis isomers are modified by changes in the pulse energy and duration.
机译:一种学习算法用于操纵光脉冲形状并优化细菌视紫红质中的视网膜异构化,激发水平高达每平方厘米1.8 x 10〜(16)个光子。低于最大激发水平的1/3,产率对脉冲形状不敏感。在此级别之上,学习算法发现傅立叶变换限制(TL)脉冲使13-顺式总体最大化。对于此最佳脉冲,屈服强度随强度线性增加,远远超过第一激发态的饱和度。为了理解这些结果,我们进行了系统的搜索,改变了泵浦脉冲的the和能量,同时监测了异构化产率。解释结果包括1光子和多光子跃迁的影响。通过改变脉冲能量和持续时间,可以改变每个中间构象中的种群动态以及全反式和13-顺式异构体之间的最终支化比。

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