...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The archaeal cofactor F_0 is a light-harvesting antenna chromophore in eukaryotes
【24h】

The archaeal cofactor F_0 is a light-harvesting antenna chromophore in eukaryotes

机译：古细菌辅因子F_0是真核生物中的光收集天线发色团

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Archae possess unique biochemical systems quite distinct from the pathways present in eukaryotes and eubacteria. 7,8-Dimethyl-8-hydroxy-5deazaflavin (F_o) and F_(420) are unique deazaflavin-contain-ing coenzyme and methanogenic signature molecules, essential for a variety of biochemical transformations associated with methane biosynthesis and light-dependent DNA repair. The deazaflavin cofactor system functions during methane biosynthesis as a low-potential hydrid shuttle F_(420)/F_(420)H_2. In DNA photolyase repair proteins, the deazaflavin cofactor is in the deprotonated state active as a light-collecting energy transfer pigment. As such, it converts blue sunlight into energy used by the proteins to drive an essential repair process. Analysis of a eukaryotic (6-4) DNA photolyase from Drosophila melanogaster revealed a binding pocket, which tightly binds F_0. Residues in the pocket activate the cofactor by deprotonation so that light absorption and energy transfer are switched on. The crystal structure of Fo in complex with the D. melanogaster protein shows the atomic details of Fo binding and activation, allowing characterization of the residues involved in F_0 activation. The results show that the F_0/F_(420) coenzyme system, so far believed to be strictly limited to the archael kingdom of life, is far more widespread than anticipated. Analysis of a D. melanogaster extract and of a DNA photolyase from the primitive eu-karyote Ostreococcus tauri provided direct proof for the presence of the F_0 cofactor also in higher eukaryotes.
机译：古细菌拥有独特的生化系统，与真核生物和真细菌的存在途径截然不同。 7,8-二甲基-8-羟基-5脱氮黄素（F_o）和F_（420）是独特的含脱氮黄素的辅酶和产甲烷标记分子，对于与甲烷生物合成和光依赖性DNA修复相关的各种生化转化至关重要。脱氮黄素辅助因子系统在甲烷生物合成过程中以低电位氢化物穿梭F_（420）/ F_（420）H_2的形式起作用。在DNA光裂解酶修复蛋白中，脱氮黄素辅助因子处于去质子化状态，具有聚光能量转移颜料的作用。这样，它会将蓝色的阳光转化为蛋白质所使用的能量，以驱动必要的修复过程。对果蝇果蝇的真核（6-4）DNA光解酶的分析显示了一个结合袋，该结合袋紧密结合F_0。口袋中的残留物通过去质子化激活了辅因子，从而开启了光吸收和能量转移。与黑腹果蝇蛋白质复合的Fo晶体结构显示了Fo结合和激活的原子细节，从而可以表征参与F_0激活的残基。结果表明，迄今为止一直认为F_0 / F_（420）辅酶系统严格限于生命的古国，其作用远比预期的要广泛。对黑腹果蝇提取物和原始真核生物真金葡菌的DNA光解酶的分析提供了F_0辅因子在高级真核生物中的存在的直接证据。

著录项

  • 来源
  • 作者

    Andreas F. Glas; Melanie J. Maul; Max Cryle; Thomas R. M. Barends; Sabine Schneider; Emine Kaya; lime Schlichting; Thomas Carell;

  • 作者单位

    Centre for Integrative Protein Science, Department of Chemistry and Biochemistry, Ludwig-Maximilians University Munich, Butenandtstrasse 5-13, 81377 Munich, Germany;

    Centre for Integrative Protein Science, Department of Chemistry and Biochemistry, Ludwig-Maximilians University Munich, Butenandtstrasse 5-13, 81377 Munich, Germany;

    Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany;

    Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany;

    Centre for Integrative Protein Science, Department of Chemistry and Biochemistry, Ludwig-Maximilians University Munich, Butenandtstrasse 5-13, 81377 Munich, Germany;

    Centre for Integrative Protein Science, Department of Chemistry and Biochemistry, Ludwig-Maximilians University Munich, Butenandtstrasse 5-13, 81377 Munich, Germany;

    Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany;

    Centre for Integrative Protein Science, Department of Chemistry and Biochemistry, Ludwig-Maximilians University Munich, Butenandtstrasse 5-13, 81377 Munich, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    crystal structure; deazaflavin; DNA photolesion; DNA repair; photolyase;

    机译：晶体结构脱氮黄素;DNA光损伤;DNA修复;光裂解酶;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号