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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A molecular brake, not a clutch, stops the Rhodobacter sphaeroides flagellar motor
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A molecular brake, not a clutch, stops the Rhodobacter sphaeroides flagellar motor

机译：分子制动器，而不是离合器，使球形红球菌鞭毛马达停止运转

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摘要

Many bacterial species swim by employing ion-driven molecular motors that power the rotation of helical filaments. Signals are transmitted to the motor from the external environment via the chemotaxis pathway. In bidirectional motors, the binding of phos-phorylated CheY (CheY-P) to the motor is presumed to instigate conformational changes that result in a different rotor-stator interface, resulting in rotation in the alternative direction. Controlling when this switch occurs enables bacteria to accumulate in areas favorable for their survival. Unlike most species that swim with bidirectional motors, Rhodobacter sphaeroides employs a single stop-start flagellar motor. Here, we asked, how does the binding of CheY-P stop the motor in R. sphaeroides-using a clutch or a brake? By applying external force with viscous flow or optical tweezers, we show that the R. sphaeroides motor is stopped using a brake. The motor stops at 27-28 discrete angles, locked in place by a relatively high torque, approximately 2-3 times its stall torque.
机译：许多细菌通过使用离子驱动的分子马达来游泳，该分子马达为螺旋丝的旋转提供动力。信号通过趋化途径从外部环境传输到电机。在双向电动机中，假定磷酸化的CheY（CheY-P）与电动机的结合会引起构象变化，从而导致不同的转子-定子界面，从而导致沿其他方向旋转。控制何时发生这种切换，使细菌能够在有利于其生存的区域积聚。与大多数使用双向马达游泳的物种不同，球形球形红细菌采用单停止-启动鞭毛马达。在这里，我们问，通过离合器或制动器，CheY-P的结合如何使红球菌中的电动机停止运转？通过用粘性流或光学镊子施加外力，我们表明球形红斑狼疮马达是通过制动器停止的。电机以27-28个离散角度停止，并通过相对较高的转矩将其锁定在适当的位置，约为其失速转矩的2-3倍。

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    Teuta Pilizota; Mostyn T. Brown; Mark C. Leake; Richard W. Branch; Richard M. Berry; Judith P. Armitage;

  • 作者单位

    The Clarendon Laboratory, Department of Physics, Oxford University, Parks Road, Oxford OX1 3PU, United Kingdom Carl kahn Laboratory, Princeton University, Washington Road, Princeton, NJ 08544;

    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom;

    The Clarendon Laboratory, Department of Physics, Oxford University, Parks Road, Oxford OX1 3PU, United Kingdom Oxford Centre for Integrative Systems Biology (OCISB), Department of Biochemistry, University of Oxford, South Parks Road, Oxford 0X1 3QU, United Kingdom;

    The Clarendon Laboratory, Department of Physics, Oxford University, Parks Road, Oxford OX1 3PU, United Kingdom;

    The Clarendon Laboratory, Department of Physics, Oxford University, Parks Road, Oxford OX1 3PU, United Kingdom;

    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom Oxford Centre for Integrative Systems Biology (OCISB), Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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