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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Dendrites of cerebellar granule cells correctly recognize their target axons for synaptogenesis in vitro
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Dendrites of cerebellar granule cells correctly recognize their target axons for synaptogenesis in vitro

机译：小脑颗粒细胞的树突可正确识别其突触的目标轴突。

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摘要

Neural circuits are generated by precisely ordered synaptic connections among neurons, and this process is thought to rely on the ability of neurons to recognize specific partners. However, it is also known that neurons promiscuously form synapses with nonspecific partners, in particular when cultured in vitro, causing controversies about neural recognition mechanisms. Here we reexamined whether neurons can or cannot select particular partners in vitro. In the cerebellum, granule cell (GC) dendrites form synaptic connections specifically with mossy fibers, but not with climbing fibers. We cocultured GC neurons with pontine or inferior olivary axons, the major sources for mossy and climbing fibers, respectively, as well as with hippocampal axons as a control. The GC neurons formed synapses with pontine axons predominantly at the distal ends of their dendrites, reproducing the characteristic morphology of their synapses observed in vivo, whereas they failed to do so when combined with other axons. In the latter case, synaptic proteins could accumulate between axons and dendrites, but these synapses were randomly distributed throughout the contact sites, and also their synaptic vesicle recycling was anomalous. These observations suggest that GC dendrites can select their authentic partners for synaptogenesis even in vitro, forming the synapses with a GC-specific nature only with them.
机译：神经回路是由神经元之间精确排序的突触连接产生的，并且该过程被认为依赖于神经元识别特定伴侣的能力。然而，还已知神经元混杂地与非特异性伴侣形成突触，特别是在体外培养时，引起关于神经识别机制的争论。在这里，我们重新检查了神经元能否在体外选择特定的伴侣。在小脑中，颗粒细胞（GC）树突特别与长满苔藓的纤维形成突触连接，但与攀登纤维不形成突触连接。我们将GC神经元分别与脑桥或下橄榄轴突（共生苔藓和攀缘纤维的主要来源）以及海马轴突进行共培养。 GC神经元主要在树突的远端与桥脑轴突形成突触，重现了体内观察到的突触的特征形态，而当与其他轴突结合时却未能如此。在后一种情况下，突触蛋白可以在轴突和树突之间积聚，但是这些突触随机分布在整个接触部位，并且突触小泡的循环异常。这些观察结果表明，即使在体外，GC树突也可以选择其真实的伴侣进行突触形成，仅与它们形成具有GC特异性的突触。

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