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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Negative membrane curvature as a cue for subcellular localization of a bacterial protein
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Negative membrane curvature as a cue for subcellular localization of a bacterial protein

机译：负膜曲率可作为细菌蛋白亚细胞定位的线索

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摘要

Bacterial proteins often localize to distinct sites within the cell, but the primary cues that dictate localization are largely unknown. Recent evidence has shown that positive membrane curvature can serve as a cue for localization of a peripheral membrane protein. Here we report that localization of the peripheral membrane protein DivIVA is determined in whole or in part by recognition of negative membrane curvature and that regions of the protein near the N and C terminus are important for localization. DivIVA, which is a cell division protein in Bacillus subtilis, localizes principally as a ring at nascent septa and secondarily to the less negatively curved, inside surface of the hemispherical poles of the cell. When cytokinesis is prevented, DivIVA redistributes itself to, and becomes markedly enriched at, the poles. When the rod-shaped cells are converted into spheres (protoplasts) by treatment with ly-sozyme, DivIVA adopts a largely uniform distribution around the cell. Recognition of membrane curvature by peripheral membrane proteins could be a general strategy for protein localization in bacteria.
机译：细菌蛋白通常位于细胞内的不同位点，但是决定定位的主要线索在很大程度上是未知的。最近的证据表明，正膜曲率可作为外周膜蛋白定位的提示。在这里，我们报道外周膜蛋白DivIVA的定位全部或部分由负膜曲率的识别来确定，并且蛋白在N和C末端附近的区域对于定位很重要。 DivIVA是枯草芽孢杆菌中的一种细胞分裂蛋白，主要定位为新生间隔处的环，其次定位于细胞半球形极的负弯度较小的内表面。当防止胞质分裂时，DivIVA会重新分布到极点并显着富集。当通过溶菌酶处理将杆状细胞转化为球体（原生质体）时，DivIVA会在细胞周围采取大致均匀的分布。外周膜蛋白识别膜曲率可能是细菌中蛋白质定位的一般策略。

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