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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A plant-specific calreticulin is a key retention factor for a defective brassinosteroid receptor in the endoplasmic reticulum
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A plant-specific calreticulin is a key retention factor for a defective brassinosteroid receptor in the endoplasmic reticulum

机译：植物特异性钙网蛋白是内质网中油菜素类固醇受体缺陷的关键保留因子

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摘要

Mammalian calreticulin (CRT) is a multifunctional Ca~(2+)-binding protein involved in more than 40 cellular processes in various subcellular compartments, such as Ca~(2+) storage and protein folding in the endoplasmic reticulum (ER). CRT homologues were discovered in plants almost 15 years ago, and recent studies revealed that many plant species contain 2 or more CRTs that are members of 2 distinct families, the CRT1/2 family and the plant-specific CRT3 family. However, little is known about their physiological functions. Here we report ebs2 (EMS-mutagenized bri1 suppressor 2) as an allere-specific suppressor of bri1-9, a dwarf Arabidopsis mutant caused by retention of a defective brassinosteroid receptor in the ER. FBS2 encodes the Arabidopsis CRT3 that interacts with ER-localized bri1-9 in a glycan-dependent manner. Loss-of-function ebs2 mutations compromise ER retention of bri1-9 and suppress its dwarfism, whereas EBS2 over-expression enhances its dwarf phenotype. In contrast, mutations of 2 other CRTs or their membrane-localized homologues calnexins had little effect on bri1-9. A domain-swapping experiment revealed that the positively charged C-terminal tail of CRT3 is crucial for its "bri1-9-retainer" function. Our study revealed not only a functional role for a plant-specific CRT, but also functional diversity among the 3 Arabidopsis CRT paralogues.
机译：哺乳动物钙网蛋白（CRT）是一种多功能的Ca〜（2+）结合蛋白，参与各种亚细胞区室的40多个细胞过程，例如Ca〜（2+）的存储和在内质网（ER）中的蛋白折叠。在大约15年前的植物中发现了CRT同源物，最近的研究表明，许多植物物种包含2个或多个CRT，它们是2个不同的家族（CRT1 / 2家族和植物特有的CRT3家族）的成员。但是，对其生理功能知之甚少。在这里，我们报告ebs2（经EMS诱变的bri1抑制剂2）作为bri1-9的变态反应特异性抑制剂，bri9是矮人拟南芥突变体，其由有缺陷的油菜素甾体受体保留在ER中引起。 FBS2编码拟南芥CRT3，它以糖基依赖性方式与ER定位的bri1-9相互作用。功能丧失的ebs2突变会损害bri1-9的ER保留并抑制其侏儒症，而EBS2的过表达会增强其侏儒表型。相比之下，其他2个CRT的突变或其膜定位同源物钙联接蛋白对bri1-9的影响很小。域交换实验显示，带正电的CRT3 C尾尾对于其“ bri1-9-retainer”功能至关重要。我们的研究不仅揭示了植物特定CRT的功能作用，还揭示了3个拟南芥CRT旁系同源物之间的功能多样性。

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    Hua Jin; Zhi Hong; Wei Su; Jianming Li;

  • 作者单位

    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Ml 48109-1048;

    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Ml 48109-1048;

    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Ml 48109-1048;

    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Ml 48109-1048;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    BRI1; calnexin; ER chaperone; ER quality control; UGGT;

    机译：BRI1;钙结合蛋白是伴侣ER质量控制;UGGT;

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